Objective: To establish an HPLC fingerprint method for quality control of Jiangnian capsule. Method: The HPLC separation was performed on an Agilent C18 (4.6 mm×250 mm

5 μm)

using acetonitrile-0.05%H3PO4 water solution as mobile phase with flow rate of 1.0 mL·min-1. The detection wavelength was 272 nm. Column temperature was set at 35℃. Injection volume was 10 μL.The 'Similarity Evaluation System for Chromatographic Fingerprint of TCM'(2004 A edition) was used to evaluate the similarity of the 10 batches of Jiangnian capsule

and to analyze the correlations of 12 ingredients in Jiangnian capsule. Result: The similarity values of 10 batches Jiangnian capsule were between 0.97-0.99.Taking puerarin as the reference peak

28 common peaks were found

of which 23 peaks were assigned to herbs

and 4 peaks were identified. Conclusion: The method has good precision

repeatability

stability

and can be used in the comprehensive quality evaluation of Jiangnian capsule.