Experimental Study of Ailuo Kechuanning on Anti-inflammatory Mechanism in Rats with Stabilization and Acute Aggravating Period of Chronic Obstructive Pulmonary Disease

SHANG Li-zhi; XIE Wen-ying; ZHANG Liang-zhi; PAN Xiao-li; HU Wen-hao; LIU Tan

doi:10.13422/j.cnki.syfjx.2015010134

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Experimental Study of Ailuo Kechuanning on Anti-inflammatory Mechanism in Rats with Stabilization and Acute Aggravating Period of Chronic Obstructive Pulmonary Disease

更新时间：2024-01-04

- Experimental Study of Ailuo Kechuanning on Anti-inflammatory Mechanism in Rats with Stabilization and Acute Aggravating Period of Chronic Obstructive Pulmonary Disease
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 1, Pages: 134-139(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015010134
  CLC： R563.9
- Published：2015
- 稿件说明：
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SHANG Li-zhi, XIE Wen-ying, ZHANG Liang-zhi, et al. Experimental Study of Ailuo Kechuanning on Anti-inflammatory Mechanism in Rats with Stabilization and Acute Aggravating Period of Chronic Obstructive Pulmonary Disease[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 134-139.
DOI：

SHANG Li-zhi, XIE Wen-ying, ZHANG Liang-zhi, et al. Experimental Study of Ailuo Kechuanning on Anti-inflammatory Mechanism in Rats with Stabilization and Acute Aggravating Period of Chronic Obstructive Pulmonary Disease[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 134-139. DOI： 10.13422/j.cnki.syfjx.2015010134.

摘要

目的: 探讨爱罗咳喘宁方对稳定期和急性加重期慢性阻塞性肺疾病(COPD)大鼠抗炎作用及其机制。方法: 采用烟熏加滴菌制备COPD稳定期和急性加重期大鼠模型。大鼠随机分为正常组、模型组、爱罗咳喘宁低、中、高剂量组。正常组、模型组灌胃生理盐水(15.52 mL·kg-1·d-1)

爱罗咳喘宁低、中、高剂量组分别灌胃(7.75

15.52

31.04 g·kg-1·d-1)

连续14 d。酶联免疫法(ELISA)测定血清、肺泡灌洗液(BALF)和肺匀浆中可溶性细胞间黏附分子-1(sICAM-1)

白细胞介素-8(IL-8)

肿瘤坏死因子-α(TNF-α)

白细胞介素-17(IL-17)和C反应蛋白(CRP)含量

光镜观察炎细胞及肺组织。结果: ①稳定期:与正常组比较

模型组稳定期支气管肺泡灌洗液(BALF)中炎细胞总数和中性粒细胞数显著增高(均P<0.05);血清、BALF及肺匀浆中sICAM-1

TNF-α

IL-8

IL-17和CRP含量均显著高于正常组(P<0.05

P<0.01)。与稳定期模型组比较

爱罗咳喘宁中、高剂量组BALF中炎细胞计数、中性粒细胞和巨噬细胞数显著减少(P<0.05

P<0.01);中、高剂量组血清、BALF、及肺匀浆中sICAM-1

TNF-α

IL-8

IL-17和CRP含量均显著降低(P<0.05

P<0.01)。②急性加重期:急性加重期模型组、爱罗咳喘宁各剂量组相对应指标与稳定期变化趋向一致。结论: 爱罗咳喘宁对稳定期和急性加重期COPD均有抗炎作用。可能通过减少中性粒细胞和巨噬细胞数

抑制细胞因子sICAM-1

TNF-α

IL-8

IL-17和CRP的分泌而起到抗炎作用。

Abstract

Objective: To study the effect of Ailuo Kechuanning on levels of soluble intercellular adhesion molecular-1(sICAM-1)

tumor necrosis factor alpha(TNF-α)

interleukin-8(IL-8)

interleukin-17(IL-17) and C-reactive protein(CRP) in serum

bronchial alveolar lavage fluid (BALF) and lung homogenates of rats with chronic obstructive pulmonary disease (COPD). Method: The method of smoke add drops of bacteria preparation was used to rat model COPD with stabilization and acute aggravatin.the rats were randomly divided into normal control group

model group

Ailuo Kechuanning group (7.75

15.52

31.04 g·kg-1·d-1)

ELISA was used to determine the levels of sICAM-1

TNF-α

IL-8

IL-17 and CRP in serum

BALF and lung homogenates. Result: ①Stable period:the total number of neutrophils and inflammatory cells were significantly increased (P<0.01) in BALF.The levels of sICAM-1

TNF-α

IL-8

IL-17 and CRP in serum

BALF and lung homogenates of the stable period model group were significantly higher than those in normal control group. While the levels of sICAM-1

TNF-α

IL-8

IL-17 and CRP were decreased significantly in serum

BALF and lung homogenates of the rats treated with Ailuo Kechuanning(15.52 g·kg-1·d-1)than those in stable period model group.②Acute exacerbation of COPD:the obvervational index changes direction in acute exacerbation period tend to be consistent with stabilization period. Conclusion: The mechanism of Ailuo Kechuanning anti-inflammatory may reduce the number of neutrophils and macrophages

and decrease the levels of sICAM-1

TNF-α

IL-8

IL-17 and CRP.

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