Optimization of Flash Extraction Technology of Puerarin from Puerariae Lobatae Radix by Orthogonal Test

LI Bao-hong; WU Jun; CHENG Yi; TIAN Yuan; WANG Jia-ping

doi:10.13422/j.cnki.syfjx.2015020027

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Optimization of Flash Extraction Technology of Puerarin from Puerariae Lobatae Radix by Orthogonal Test

更新时间：2024-01-04

- Optimization of Flash Extraction Technology of Puerarin from Puerariae Lobatae Radix by Orthogonal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 2, Pages: 27-29(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015020027
  CLC： R284.2
- Published：2015
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LI Bao-hong, WU Jun, CHENG Yi, et al. Optimization of Flash Extraction Technology of Puerarin from Puerariae Lobatae Radix by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 27-29.
DOI：

LI Bao-hong, WU Jun, CHENG Yi, et al. Optimization of Flash Extraction Technology of Puerarin from Puerariae Lobatae Radix by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 27-29. DOI： 10.13422/j.cnki.syfjx.2015020027.

摘要

目的: 优选葛根中葛根素的闪式提取工艺。方法: 在单因素试验基础上

以葛根素提取率为评价指标

采用正交试验法考察乙醇体积分数、料液比、提取电压、提取时间对葛根中葛根素提取工艺的影响。采用HPLC测定葛根素含量

流动相甲醇-水(25:75)

检测波长250 nm。结果: 最佳提取工艺为加15倍量50%乙醇于90 V提取2 min;葛根素提取率37.42 mg ·g-1

转移率88.89%。结论: 优选的工艺稳定可行、重复性好、提取时间短

可作为葛根中葛根素的工业化生产工艺参数。

Abstract

Objective: To optimize flash extraction process of puerarin from Puerariae Lobatae Radix. Method: Based on single tests

taking extracting rate of puerarin as index

orthogonal test was adopted to optimize flash extraction process with ethanol concentration

solid-liquid ratio

extraction voltage and time as factors.HPLC was employed to determine the content of puerarin with mobile phase of methanol-water(25: 75) and detection wavelength at 250 nm. Result: Optimum extraction technology was as following:extracted 2 min with 15 times the amount of 50% ethanol at 90 V.Under these conditions

extracting rate of puerarin was 37.42 mg · g-1 and its transfer rate was 88.89%. Conclusion: This optimized process is stable and reproducible with short extracting time

it can be used as process parameters for industrial production of puerarin from Puerariae Lobatae Radix.

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Related Institution

National Key Laboratory of Creation of Modern Chinese Medicine with Classical Formulas

School of Pharmacy， Jiangxi University of Chinese Medicine

Key Laboratory of Modern Chinese Medicine Formulations of Jiangxi University of Chinese Medicine， Ministry of Education

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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