LEI Yun, WANG Wei, SHI Yang, et al. HPLC Fingerprints Integrated with Quantitative Determination of Multi-components for Quality Control of Angelicae Dahuricae Radix[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 36-39.
DOI:
LEI Yun, WANG Wei, SHI Yang, et al. HPLC Fingerprints Integrated with Quantitative Determination of Multi-components for Quality Control of Angelicae Dahuricae Radix[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 36-39. DOI: 10.13422/j.cnki.syfjx.2015020036.
HPLC Fingerprints Integrated with Quantitative Determination of Multi-components for Quality Control of Angelicae Dahuricae Radix
并同时测定欧前胡素和异欧前胡素的含量。方法: 采用Platisil DOS C18色谱柱(4.6 mm×250 mm
5 μm)
乙腈-水为流动相进行梯度洗脱
检测波长254 nm。共收集了10批白芷进行测定
运用国家药典委员会“中药色谱指纹图谱相似度评价软件”2004A版进行评价
并对2种成分进行含量测定。结果: 建立了白芷香豆素类成分HPLC指纹图谱
标定了10个共有色谱峰。含量测定条件通过方法学验证
平均加样回收率分别为102.52%
98.23%。结论: 该法所建立的白芷香豆素类成分指纹图谱特征性强、方法简便
结合欧前胡素和异欧前胡素含量测定可更好地控制其质量
对白芷的鉴定及质量控制具有指导意义和参考价值。
Abstract
Objective: A method of HPLC-DAD was developed to evaluate the quality of Angelicae Dahuricae Radix through multi-target ingredient determination and finger printing analysis technologies. Method: The optimal conditions of separation and detection were achieved on a Platisil DOS C18(4.6 mm×250 mm
5 μm) with linear gradient elution of acetonitrile-water. The detection wavelength was 254 nm. Collect 10 batches from different origins in China to assess the similarities according to similarity evaluation for ‘chromatographic fingerprint of traditional Chinese medicine’(2004A). Result: In quantitative analysis
the two components showed good regression with linear ranges
and their recoveries were 102.52% and 98.23%. Conclusion: This study indicated that the combination of quantitative and chromatographic fingerprint analysis can be readily utilized as a quality control method for Angelicae Dahuricae Radix.
Preliminary Exploration on Ultrafine Grinding Rules of Angelicae Dahuricae Radix Pieces
Effect of Single and Mixed Decoction of Angelicae Dahuricae Radix and Myrrha on Content of Imperatorin and Their Analgesic Effect
Correlation Analysis Between Microbial Community Changes and Medicinal Quality Formation During Processing of Angelicae Dahuricae Radix
Research Progress on Pharmacological Effect and Mechanism of Imperatorin
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