Antioxidant Activity of  Extracts

XU Ben-shan; LEI Ning; LYU Ya-li; MA Ping; DU Shu-shan; SHEN Jia-jia

doi:10.13422/j.cnki.syfjx.2015020061

您当前的位置：

首页 >

文章列表页 >

Antioxidant Activity of Extracts

更新时间：2024-01-04

- Antioxidant Activity of Extracts
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 2, Pages: 61-64(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015020061
  CLC： R284.1;R285.5
- Published：2015
- 稿件说明：
移动端阅览
XU Ben-shan, LEI Ning, LYU Ya-li, et al. Antioxidant Activity of Extracts [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 61-64.
DOI：

XU Ben-shan, LEI Ning, LYU Ya-li, et al. Antioxidant Activity of Extracts [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 61-64. DOI： 10.13422/j.cnki.syfjx.2015020061.

摘要

目的: 通过体外抗氧化和还原活性评价实验

研究藏药雪灵芝乙醇提取物不同极性溶剂萃取部分的抗氧化作用。方法: 用体积分数95%的乙醇回流提取雪灵芝粉末

浓缩的乙醇浸膏依次用石油醚、二氯甲烷、乙酸乙酯、正丁醇进行萃取

各部分分别配制成适当浓度的溶液

采用1

1-二苯基-2-苦基肼(DPPH)自由基法

2-连氮-双-(3-乙基苯并噻唑啉磺酸-6) 二铵盐(ABTS)自由基法和铁离子还原/抗氧化能力(FRAP)法分别测定各部分的自由基清除能力和铁离子还原力。结果: 雪灵芝醇提物的4个萃取部分均具有一定的体外抗氧化作用

其中乙酸乙酯与正丁醇部分清除DPPH ·

ABTS+ ·及还原Fe3+的能力均比二氯甲烷与石油醚部分强。结论: 雪灵芝提取物清除能力与提取物样品浓度在一定范围内具有良好的量效关系

其中极性较大的乙酸乙酯部分和正丁醇部分的抗氧化活性及还原能力相对较强。

Abstract

Objective: To investigate the antioxidant activity of Arenaria kansuensis extracts in vitro. Method: The air-dried and powderded herb of A. kansuensis was extracted over 2 hours with 10 times of 95% alcohol refluxing for 2 times and the combined alcohol extracts were concentrated under vacuum drying. The resultant residue was partitioned in petroleum ether

dichloromethane

ethyl acetate and n-butanol orderly. The four extracts were configured to the appropriate solution. The antioxidant activity of four extracts were analyzed by 1

1-diphenyl-2-pieryl-2-picrylhydrazyl (DPPH) radical scavenging

2-amino-di(3-ethyl-benzothiazoline sulphonic acid-6) ammonium salt (ABTS) radical scavenging

and ferric reducing antioxidant power (FRAP) assay. Result: The ethyl acetate and n-butanol extract of A. kansuensis showed stronger scavenging activity against DPPH ·

ABTS+ · and reducing antioxidant power of 2

6-tris (2-pyridyl) -s-triazine(TPTZ) than other extracts

but showed lower capacity than positive control of vitamin C. Conclusion: The scavenging effects showed a dose-response relationship at the selected concentration with the extracts of A. kansuensis. The ethyl acetate and n-butanol extract have the strongest free radical scavenging activity.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Removing Core on Quality of Phyllanthi Fructus

Study on Extraction Technology and Antioxidant Activity of Theabrownine from Oolong Tea

Dose-response Relationship of Free Radical Scavenging Activity by Blood-activating and Stasis-removing Formulae

Relationship Between Total Flavonoids, Total Phenolics and 2,2-diphenyl-1-picrylhydrazyl Radical Scavenging Activities during Growth of Leaves

Antioxidant Activity of 3-bromo-4,5-dihydroxybenzaldehyde

Related Author

Chuan-hong LUO

Sheng-jie HUANG

Qi-qi HU

Peng TAN

Hua-mei GOU

Xi-chuan WEI

Hao-zhou HUANG

San-hu FAN

Related Institution

School of Pharmacy，State Key Laboratory of Characteristic Chinese Drug Resources in Southwest China，Chengdu University of Traditional Chinese Medicine（TCM）

State Key Laboratory of Biological Evaluation of TCM Quality，National Administration of TCM， Sichuan Academy of Chinese Medicine Sciences

Sanajon Pharmaceutical Group

Teaching Hospital of Chengdu University of TCM

College of Chemistry,Chemical Engineering and Environment,Qingdao University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰