Chemical Constituents and Antioxidant Evaluation of

MA Ying; GAO Ting-ting; TIAN Li-ping; TAN Yong; WANG Jin-hui

doi:10.13422/j.cnki.syfjx.2015020070

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Chemical Constituents and Antioxidant Evaluation of

更新时间：2024-01-04

- Chemical Constituents and Antioxidant Evaluation of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 2, Pages: 70-73(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015020070
  CLC： R284.1
- Published：2015
- 稿件说明：
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MA Ying, GAO Ting-ting, TIAN Li-ping, et al. Chemical Constituents and Antioxidant Evaluation of [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 70-73.
DOI：

MA Ying, GAO Ting-ting, TIAN Li-ping, et al. Chemical Constituents and Antioxidant Evaluation of [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 70-73. DOI： 10.13422/j.cnki.syfjx.2015020070.

摘要

目的: 研究窄叶芍药的化学成分

并对分离得到的化合物进行抗氧化活性评价。方法: 采用反复硅胶柱色谱法和Sephadex LH-20 柱色谱法等进行分离纯化

并通过理化常数测定和光谱分析鉴定其化学结构;并采用1

1-二苯基-2-硝基苯肼自由基清除法(DPPH)和2

2-联氮基-双-(3-乙基苯并噻唑啉磺酸-6)二铵盐自由基清除法(ABTS)对化合物的体外活性进行评价。结果: 从窄叶芍药中分离得到5个化合物

并鉴定为没食子酸乙酯(1)

没食子酸甲酯(2)

齐墩果酸(3)

丁香酸(4)

β-谷甾醇(5);对化合物 1~4 进行抗氧化活性测试

结果表明:阳性药(维生素C

VC)及化合物 1 和 2 对DPPH自由基的半数清除率(IC50)分别达到(35.13±0.28)

(44.64±0.37) μmol ·L-1

均低于阳性对照抗坏血酸IC50(60.17±0.98) μmol ·L-1;阳性药及化合物 1 和 2 对ABTS自由基的IC50分别达到(25.13±0.68)

(35.76±1.48) μmol ·L-1

均低于阳性对照抗坏血酸IC50(38.55±0.78) μmol ·L-1。结论: 化合物 3

4 首次从该植物中分离得到

并且化合物 1 和化合物 2 具有很强的抗氧化活性

其余化合物的抗氧化活性较弱。

Abstract

Objective: To study the chemical constituents from Paeonia anomala and to evaluate their antioxidant activities. Method: The compounds were isolated by chromatography on silicagel

Sephadex LH-20 column and identified on the basis of physicochemical constants and spectral analysis. Their antioxidant activities were evaluated by 1

1-diphenyl-2-pieryl-2-picrylhydrazyl(DPPH) and 2

2-amino-di(3-ethyl-benzothiazoline sulphonic acid-6) ammonium salt (ABTS) radical scavenging methods. Result: Five compounds were isolated and their structures were identified as ethyl gallate (1)

methyl gallate (2)

oleanic acid (3)

syringate (4) and β-sitosterol(5). The results showed that the DPPH radical scavenging activity of compounds 1 and 2 were lower than the positive control of Vitamin C with IC50values (35.13±0.28)

(44.64±0.37) μmol · L-1. Their ABTS radical scavenging activity were lower than Vitamin C with IC50values of (25.13±0.68)

(35.76±1.48) μmol · L-1

respectively. Conclusion: Compounds 3

4 are isolated from this plant for the first time. Compounds 1

2 have powerful antioxidant activies

and the others have little antioxidant activies.

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