Objective: In Xintongning spray quality standard

it had the determination of requirements on the ferulic acid and cinnamic acid content

but for two sets of different methods.The method established a HPLC method for the determination of ferulic acid

cinnamic acid in Xintongning spray. Method: The method used high performance liquid chromatography separation and the effective components of the calculation of its content.The analysis was performed on a Phenomenex Luna C18 column(4.6 mm×150 mm

5 μm)

mobile phase was acetonitrile -0.05% phosphoric acid for gradient elution

the detection wavelength was 290 mm

the flow rate was 1.0 mL · min-1 and column temperature at 30 ℃. Selected the 1 batch of Xintongning spray samples for method validation test

and the 4 batch of Xintongning spray samples according to the method of content determination. Result: Blank interference test

precision test

stability test

repeatability test

linear relation test

recovery test method validation tests were in line with the relevant requirements

RSD was less than 3%.The linearities were in the ranges of 0.025 16-0.276 76 mg · L-1 for fetulic acid(r =1)

0.010 4-0.114 4 mg · L-1 for cinnamic acid(r =0.999 5)

respectively. The two components were able to achieve baseline separation

separation degree greater than 2.The average recoveries were 100.35%

101.93% for ferulic acid

cinnamic acid. Conclusion: The method is simple

accurate

reproducible

can be used for quality control of this preparation

and can be saved testing time and the testing cost objective. The method for the quality standard of Chinese traditional patent medicine multi group method for the determination of content of the establishment has reference significance.