NIU Xiao-jing, LU Jing, DUAN Xiao-ying, et al. Determation of Liquiriti, Naringin, Hesperidin, Neohesperidin and Glycyrrhizic Acid Ammonium Salt in Jianpi Shuwei Gel by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 77-79.
DOI:
NIU Xiao-jing, LU Jing, DUAN Xiao-ying, et al. Determation of Liquiriti, Naringin, Hesperidin, Neohesperidin and Glycyrrhizic Acid Ammonium Salt in Jianpi Shuwei Gel by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 77-79. DOI: 10.13422/j.cnki.syfjx.2015020077.
Determation of Liquiriti, Naringin, Hesperidin, Neohesperidin and Glycyrrhizic Acid Ammonium Salt in Jianpi Shuwei Gel by HPLC
Objective: To develop a method for determining liquiriti
naringin
hesperidin
neohesperidin and glycyrrhizic acid ammonium salt in Jianpi Shuwei gel. Method: The mobile phase consisted of acetonitrile-0.05% phosphoric acid with gradient elution at the flow rate of 1.0 mL · min-1.The detection wavelength was 237 nm(liquiriti
glycyrrhizic acid ammonium salt)
283 nm(naringin
hesperidin
neohesperidin). Result: The liquiriti
naringin
hesperidin
neohesperidin and glycyrrhizic acid ammonium salt all had good linear relationship in the ranges of 0.128 3-0.641 6
1.105 2-5.526
0.225 2-1.126 0
1.092-5.460
0.314 9-1.574 4 μg.The average recoveries were 101.47%
99.22%
100.02%
101.64% and 98.11%(RSD<3%)
respectively. Conclusion: The method is simple
accurate
reproducible and nice to the separation
can be used for the quality evaluation of Jianpi Shuwei gel.
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Related Author
GUO Qi-li
LU Wu-qing
ZENG Li-ping
LI Cai-tang
Jing-jing LIU
Xiao-ru HU
Yi HE
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Related Institution
National Institutes for Food and Drug Control
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Jiangxi University of Traditional Chinese Medicine
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