WANG Li-ping, CAO Ying-jie, DUAN Xiao-ying. Simultaneous Determination of Six Constituents in Gengnianshu Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 83-86.
DOI:
WANG Li-ping, CAO Ying-jie, DUAN Xiao-ying. Simultaneous Determination of Six Constituents in Gengnianshu Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 83-86. DOI: 10.13422/j.cnki.syfjx.2015020083.
Simultaneous Determination of Six Constituents in Gengnianshu Pills by HPLC
Objective: To establish an HPLC method for determination of gallic acid
loganin
puerarin
ferulic acid
emodin and physcion in Gengnianshu pills. Method: HPLC was applied on an Agilent C18 column (Zorbax SB-AQ 4.6mm×250 mm
5 μm) with acetonitrile-0.2% formic acid water as mobile phase by gradient elution. The flow rate was 1.0 mL·min-1. Detection wavelengthes were set at 238 nm (loganin)
270 nm (gallic acid
puerarin
emodin and physcion)
and 320 nm (ferulic acid). Result: The methodology of 6 components met the requirements
the blank test showed no interference
the average recovery rates were 98.2%-103.2%
and RSD was 1.4%-1.9%. Conclusion: This method is easy
accurate with high specificity. This study provides scientific basis for the quality evaluation of Gennianshu pills.