Objective: To simultaneous determine the contents of rhein

emodin and chrysophanol in Wenshen Jiangzhuo powder by HPLC. Method: An external standard method by HPLC with ANGLA Promosil C18 column (4.6 mm×250 mm

5 μm) was used with the column temperature was 30 ℃;the detection wavelength was 224 nm

and the injection volume was 10 μL. The flow rate was set at 1.0 mL · min-1 and gradient elution with acetonitrile and H2O (included 0.1% phosphoric acid). Result: Rhein

emodin and chrysophanol were well separated by this method. Linearities of rhein

emodin and chrysophanol were good in the ranges of 0.757 2-3.786 0 μg(r=0.999 4)

0.347 6-1.738 0 μg(r=0.999 3)

and 1.731-8.656 μg(r=0.999 2)

respectively. The average recoveries of rhein

emodin and chrysophanol were 101.7%

102.6% and 101.1%

RSD were 3.0%

2.3%

1.9%. Conclusion: The method is simple

accurate and reproducible

and can be used for quality control of Wenshen Jiangzhuo powder.