Objective: To established an UPLC-MS method for quantitative determination of six active ingredients in Shenqi Yiqi Guben tablet. Method: The UPLC separation was carried out with an Acquility UPLC BEH C18(2.1 mm×100 mm

1.7 μm) using gradient elution with a mobile phase consisting of acetonitrile-water (included 0.1% formic acid) at a constant flow rate of 0.5 mL · min-1. The MS detector set as follows:negative ion mode detection

source spray voltage (IS)-5 000 V

auxiliary heating gas temperature 450 ℃

atomization gas 50 psi

auxiliary gas 40 psi

curtain gas 30 psi

deaggregation voltage -55 V

collection methods TOF-MS/MS. Result: Six components:paeoniflorin

ginsenoside Re

ginsenoside Rg1

forsythin

ginsenosides Rb1

and astragaloside were in a good linearity

r greater than 0.999

recoveries were 98%-102%

RSD were less than 4.5%. Conclusion: The established method for the determination of the six components of Shenqi Yiqi Guben tablet is accuracy and precision that can be used for quality control of the preparation. The UPLC-MS method can increased resolution

lower production costs of drugs compared with HPLC-MS. It provides a reference to set standards for other Chinese medicine formulations containing these ingredients.