Effect of Chaihu Shugan Decoction on Content of 5-HT and Expression of IL-1 mRNA IL-6 mRNA of Hippocampus in Chronic Temporal Lobe Epilepsy-deoression Modle Rats

REN Zhi-jun; XIE Wei; LIU Yuan-zhen; ZHENG Yue-hui

doi:10.13422/j.cnki.syfjx.2015020115

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Effect of Chaihu Shugan Decoction on Content of 5-HT and Expression of IL-1 mRNA IL-6 mRNA of Hippocampus in Chronic Temporal Lobe Epilepsy-deoression Modle Rats

更新时间：2024-01-04

- Effect of Chaihu Shugan Decoction on Content of 5-HT and Expression of IL-1 mRNA IL-6 mRNA of Hippocampus in Chronic Temporal Lobe Epilepsy-deoression Modle Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 2, Pages: 115-119(2015)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.2015020115
  CLC： R285.5
- Published：2015
- 稿件说明：
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REN Zhi-jun, XIE Wei, LIU Yuan-zhen, et al. Effect of Chaihu Shugan Decoction on Content of 5-HT and Expression of IL-1 mRNA IL-6 mRNA of Hippocampus in Chronic Temporal Lobe Epilepsy-deoression Modle Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 115-119.
DOI：

REN Zhi-jun, XIE Wei, LIU Yuan-zhen, et al. Effect of Chaihu Shugan Decoction on Content of 5-HT and Expression of IL-1 mRNA IL-6 mRNA of Hippocampus in Chronic Temporal Lobe Epilepsy-deoression Modle Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 115-119. DOI： 10.13422/j.cnki.syfjx.2015020115.

摘要

目的: 观察柴胡疏肝汤对慢性颞叶癫痫-抑郁共病模型大鼠海马中5-羟色胺(5-HT)含量及白介素-1β(IL-1β)

白介素-6(IL-6)mRNA表达的影响。方法: 腹腔注射氯化锂-匹罗卡品制作癫痫-抑郁共病大鼠模型

将成功模型随机分为模型组

西酞普兰组

柴胡疏肝汤高、中、低剂量组。正常组10只。药物干预组分别给予西酞普兰10 mg ·kg-1 ·d-1

柴胡疏肝汤高、中、低剂量10

2.5 g ·kg-1 ·d-1

正常组与模型组对应给予等量生理盐水ig

连续4周

2次/d。通过强迫游泳(FST)和蔗糖消耗试验评估抑郁行为的变化;视频录像监测大鼠痫性发作次数;液相色谱-质谱法检测海马5-HT的含量;实时荧光定量PCR检测海马IL-1β

IL-6 mRNA的表达量。结果: 与正常组比较

模型组大鼠FST累计不动时间明显延长

IL-1β mRNA表达量明显增加

糖水消耗量明显减少(P<0.01)

5-HT含量减少

IL-6 mRNA表达量增加(P<0.05)。与模型组比较

药物干预4周后

西酞普兰

柴胡疏肝汤高、中剂量组大鼠痫性发作次数明显减少

FST累计不动时间明显缩短

糖水消耗量明显增加

海马中IL-1β

IL-6 mRNA的表达明显下调(P<0.01﹚;5-HT的含量增加(P<0.05﹚。西酞普兰组与柴胡疏肝汤高、中剂量组比较

各指标差异均无统计学意义。结论: 柴胡疏肝汤高、中剂量具有抑制慢性颞叶癫痫-抑郁共病模型大鼠海马中IL-1β

IL-6 mRNA表达

促进5-HT表达

减少大鼠痫性发作次数

改善抑郁行为的作用。

Abstract

Objective: To observe the effect of Chaihu Shugan decoction (CHSGD) on the content of serotonin(5-HT)

interleukin-1beta(IL-1β)

interleukin-6(IL-6)mRNA expression of hippocampus in chronic temporal lobe epilepsy and depression model rats. Method: The intraperitoneal injection of lithium chloride-pilocarpine was used to make epileptic-depression model

the modeling rats were randomly divided into model group

citalopram group

CHSGD-high

medium

low dose group and normal group. The drug intervention group was given citalopram 10 mg · kg-1 · d-1

CHSGD-H

L at dose of 10

2.5 g · kg-1 · d-1 orally

normal group and model group were given equal salineby continuous lavage for 4 weeks

twice each day. By forced swim test (FST) and the changes of sucrose consumption the depressive behavior was assessed

video was usec to monitor seizures

hippocampal 5-HT number was determined by liquid chromatography mass spectrometry. Hippocampal IL-1β

IL-6 mRNA expression was detected by real-time fluorescence quantitative PCR detection. Result: Compared with normal group

model group rats FST total fixed time extended obviously

the sugar water consumption decreased significantly

IL-1β mRNA expression quantity increased obviously (P<0.01);5-HT content reduced

IL-6 mRNA expression increased (P<0.05). Compared with model group

drug intervention after 4 weeks

citalopram

CHSGT-H

M dose group of seizures in rats reduced significantly

FST total fixed time shortened significantly

sugar water consumption increased significantly

the hippocampus IL-1β

IL-6 mRNA expression lowered significantly (P<0.01)

the content of 5-HT increase (P<0.05). Citalopram group and CHSGD-H

M dose group of comparison

there was no statistical significance. Conclusion: High

medium dose of Chaihu Shugan decoction can inhibit epilepsy comorbidity of depression in hippocampus of modle rats IL-1β

IL-6 mRNA expression

promote the expression of 5-HT

reduce the number of seizures and improve depressive behavior in rats.

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