CHEN Xiao-ting, GE Xin-yu, LIU Bin, et al. Effects of Xihuang Wan Drug Serum on Cell Migration and Invation in SW480 Human Colorectal Cancer Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 136-141.
DOI:
CHEN Xiao-ting, GE Xin-yu, LIU Bin, et al. Effects of Xihuang Wan Drug Serum on Cell Migration and Invation in SW480 Human Colorectal Cancer Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 136-141. DOI: 10.13422/j.cnki.syfjx.2015020136.
Effects of Xihuang Wan Drug Serum on Cell Migration and Invation in SW480 Human Colorectal Cancer Cells
Objective: To investigate the effects and potential mechanisms of Xihuang Wan on cell migration and invasion in SW480 human colorectal cancer cells. Method: SW480 human colorectal cancer cells were used as the cell model. Wound healing and transwell assay were used to determine the inhibitory effects of serum of rats containing Xihuang Wan on cell migration and invasion in SW480 cells
respectively. Western blot was used to measure the protein expression levels of ERK and phospho-ERK
and the qPCR assay was used to evaluate the mRNA expression levels of E-cadherin
PARP-1
Vimentin and Snail in SW480 cells after serum containing Xihuang Wan treatment. Result: The abilities of cells migration and invasion showed dose-dependent reducing in SW480 cells after treatments with the serums containing Xihuang Wan. The qPCR results showed that the serum containing Xihuang Wan enhanced E-cadherin
while reduced PARP-1
Vimentin and Snail mRNA expression levels. In addition
Western blot results showed the decreasing phosphorylation of ERK after treatments with serum containing Xihuang pill. Conclusion: The serums containing Xihuang Wan inhibited cells migration and invasion
affected the mRNA expression levels of epithelial-mesenchymal transiton (EMT) related markers including E-cadherin
PARP-1
Vimentin and Snail in SW480 cells. The mechanism may be related to the ERK/MAPK pathway. Our studies provide further pharmacological basis for developing Xihuang Wan as a preventive/chemotherapeutic agent of metastasis in colorectal cancer.
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