SHI Zhao-ping, ZHOU Xiao-hui, XU Qian, et al. Effect of Paeonol on Smad2, Smad3, Smad7 mRNA Expression in Acute Myocardial Infarction Rat Heart[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 146-150.
DOI:
SHI Zhao-ping, ZHOU Xiao-hui, XU Qian, et al. Effect of Paeonol on Smad2, Smad3, Smad7 mRNA Expression in Acute Myocardial Infarction Rat Heart[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 146-150. DOI: 10.13422/j.cnki.syfjx.2015020146.
Effect of Paeonol on Smad2, Smad3, Smad7 mRNA Expression in Acute Myocardial Infarction Rat Heart
Objective: To discuss the protective effect of paeonol on ventricular remodeling in acute myocardial infarction (AMI) rats and to investigate its influence on Smad2
Smad3
Smad7 mRNA expression of TGF-β/Smads signaling pathway. Method: In vivo model of AMI was made using the left anterior descending coronary branch ligation. The rats were randomly divided into the model group
the sham group
the low-
medium-and high-dose paeonol groups (8
12
16 mg · kg-1)
and the captopril group (10 mg · kg-1) based on ECG evaluation. The heart weight index and infarct size were measure after 4 weeks. The mRNA expression levels of Smad2
Smad3
Smad7 in myocardial tissues were detected using real-time quantitative (RT-qPCR) technique. Result: Compared with the model group
the myocardial infarct size was significantly reduced (P<0.05)
the heart weight index decreased (P<0.05) in all dose paeonol and captopril groups. Compared with the sham group
Smad2 and Smad3 mRNA expression levels were significantly higher
Smad7 expression level was lower in the model group (P<0.01). While
Smad2 and Smad3 mRNA expression levels decreased significantly
Smad7 expression level increased significantly in all dose paeonol groups (P<0.01). Conclusion: Paeonol has anti-myocardial fibrosis effect in AMI rats. Its mechanism may be related to increasing the Smad7 mRNA expression of TGF-β1 inhibitory signaling protein
and down-regulating the Smad2 and Smad7 mRNA expressions of TGF-β receptor signaling protein.
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