Influence of Different Effective Extracts of Mori Folium on HepG2 Cells

HE Xian-xia; CHANG Hua-jing; HAI Yang; WU Xin-rong

doi:10.13422/j.cnki.syfjx.2015020192

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Influence of Different Effective Extracts of Mori Folium on HepG2 Cells

更新时间：2024-01-04

- Influence of Different Effective Extracts of Mori Folium on HepG2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 2, Pages: 192-195(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015020192
  CLC： R285.5
- Published：2015
- 稿件说明：
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HE Xian-xia, CHANG Hua-jing, HAI Yang, et al. Influence of Different Effective Extracts of Mori Folium on HepG2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 192-195.
DOI：

HE Xian-xia, CHANG Hua-jing, HAI Yang, et al. Influence of Different Effective Extracts of Mori Folium on HepG2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 192-195. DOI： 10.13422/j.cnki.syfjx.2015020192.

摘要

目的: 探讨桑叶生物碱、黄酮及多糖对HepG2细胞胰岛素抵抗的改善及其作用机制。方法: 采用高糖高胰岛素培养基诱导HepG2细胞形成红外(IR)模型

葡萄糖氧化酶法检测正常组、模型组、罗格列酮组、生物碱组、黄酮组及多糖组的细胞葡萄糖消耗情况

并用RT-qPCR法检测C-Jun氨基端激酶(JNK)通路相关基因的表达

观察桑叶生物碱、黄酮及多糖改善HepG2细胞胰岛素抵抗及其作用机制。结果: 高糖及高胰岛素培养基培养 HepG2 细胞

使细胞葡萄糖消耗率明显下降

造成胰岛素抵抗的细胞模型

给予桑叶生物碱、黄酮及多糖干预之后

细胞葡萄糖消耗率明显上升

同时下调JNK和上调IRS-1

AKT mRNA 的表达。结论: 桑叶生物碱、黄酮及多糖均可增加HepG2细胞的葡萄糖消耗量

其改善胰岛素抵抗作用可能与调节JNK信号通路有关。

Abstract

Objective: To investigate the protective effect and its mechanism of different effective extracts of Mori Folium on the infrared(IR) model of HepG2 cells. Method: High glucose and insulin in HepG2 cells to form a cell model of insulin resistance

glucose oxidase detect the expression of glucose consumption

and using the real-time fluorescent quantitative polymerase chain reaction(PCR) technique to observe the expression level of C-Jun amino termind kinose(JNK) pathway related genes in different effective extracts of Mori Folium on the IR model of HepG2 cells. Result: High sugar and high insulin culture environment capable of inducing cells to produce insulin resistance.All of the groups can increase the glucose consumption(P<0.01)and has the influence on the expression of JNK related genes. Conclusion: The alkaloids

flavonoids and polysaccharides of mulberry leves can increase the glucose consumption on the HepG2 cells

which may be related to regulating JNK signaling pathways.

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