Optimization of Purification Technology of Total Alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 Macroporous Resin

CHEN Xiao-bin; ZHOU Qin-mei; LIU Shun; GUAN Min; GHENG Ge

doi:10.13422/j.cnki.syfjx.2015030021

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Optimization of Purification Technology of Total Alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 Macroporous Resin

更新时间：2024-01-04

- Optimization of Purification Technology of Total Alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 3, Pages: 21-23(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015030021
  CLC： R284
- Published：2015
- 稿件说明：
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CHEN Xiao-bin, ZHOU Qin-mei, LIU Shun, et al. Optimization of Purification Technology of Total Alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 21-23.
DOI：

CHEN Xiao-bin, ZHOU Qin-mei, LIU Shun, et al. Optimization of Purification Technology of Total Alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 21-23. DOI： 10.13422/j.cnki.syfjx.2015030021.

摘要

目的: 优选D101型大孔树脂纯化山豆根总生物碱的工艺条件。方法: 选择苦参碱为指标成分

采用UV测定总生物碱含量

检测波长415 nm。以总生物碱含量为指标

通过单因素试验考察上样量质量浓度、洗脱剂用量及种类、洗脱流速等因素对山豆根总生物碱大孔树脂纯化工艺的影响。结果: 最佳工艺条件为上样液用盐酸调pH 3~4

用20%氨水调pH 10

上样液质量浓度0.8 g·mL-1

上样流速2 BV·h-1

上样量为山豆根药材与树脂体积比例1:2.5

加水2 BV除杂

用70%乙醇4 BV于4 BV·h-1流速洗脱;总生物碱纯度66.83%。结论: D101型大孔树脂对山豆根生物碱类物质具有较好的纯化效果

优选的工艺简单可行

为山豆根的资源利用提供参考

Abstract

Objective: To optimize purification technology conditions of total alkaloids from Sophorae Tonkinensis Radix et Rhizoma by D101 macroporous resin. Method: Taking matrine as index component

UV was employed to determine the content of total alkaloids with detection wavelength of 415 nm.With the content of total alkaloids as index

effects of the concentration of sample solution

amount and type of eluent

elution velocity on purification process were investigated by single factor tests. Result: Optimum technology conditions were as follows:adjusted pH of sample solution to 3-4 with with hydrochloric acid

adjusted pH to 10 with 20% ammonia water

the concentration of sample solution 0.8 g·mL-1

sample flow rate of 2 BV·h-1

sample volume(equivalent as crude drug)-resin volume ratio of 1: 2.5

washed impurity with 2 BV of water

with 4 BV of 70% ethanol as eluant

elution flow rate of 4 BV·h-1.Purity of total alkaloids was 66.83%. Conclusion: D101 macroporous resin has good purification effect for total alkaloids from Sophorae Tonkinensis Radix et Rhizoma

optimized process is simple and feasible

this study provides a reference for resource utilization of Sophorae Tonkinensis Radix et Rhizoma.

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Related Institution

National Institutes for Food and Drug Control

School of TCM， Beijing University of Chinese Medicine

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National Engineering Laboratory for Quality Control Technology of Chinese Materia Medica， Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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