WANG Han-yuan, DONG Jin-xiang, DONG Xue-lian, et al. Optimization of Purification Technology for Total Flavonoids from Pyrrosiae Folium by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 27-29.
DOI:
WANG Han-yuan, DONG Jin-xiang, DONG Xue-lian, et al. Optimization of Purification Technology for Total Flavonoids from Pyrrosiae Folium by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 27-29. DOI: 10.13422/j.cnki.syfjx.2015030027.
Optimization of Purification Technology for Total Flavonoids from Pyrrosiae Folium by Macroporous Resin
Objective: To optimize purification process of total flavonoids from Pyrrosiae Folium by macroporous resin. Method: The content of total flavonoids was determined by UV-visible spectrophotometry
detection wavelength was 510 nm.With the content of total flavonoids as index
types of macroporous resin were screened by static adsorption-elution test
single factor tests were adopted to optimize purification technology with sample volume
ratio of diameter to height
the amount of eluent and so on. Result: D101 macroporous resin showed better adsorption-elution effect than others
optimum purification technology was as following:height-diameter ratio of 6-10
ratio of resin to crude drug 1: 2.73
the concentration of sample solution 0.5 g·mL-1
absorption velocity 2 BV·h-1
washed impurity with 8 BV of water
then eluted with 5 BV of 60% ethanol at 4 BV·h-1
collected eluent.Under these conditions
purity of total flavonoids was 59.06%. Conclusion: D101 macroporous resin has good purification effects on total flavonoids from Pyrrosiae Folium
this optimized purification technology is reasonable