Objective: The current study aimed to identify the chemical constituents occurring in the leaves of Alpinia officinarum. Method: The collected leaves of A. officinarum were dried in an electric thermostatic drying oven at 40°C overnight. The freshly dried leaves were smashed using a universal high-speed smashing machine and then sieved manually by an 80 mesh. The resulting fine powders and residue were mixed evenly. An aliquot (0.6 g) was weighed precisely and macerated with 30 mL of methanol and then ultrasonicated three times for 30 min each. For each ultrasonication extraction

the resulting extract solutions were centrifuged at 8 000 rpm for 10 min. One micro liter of supernatant was sampled and the remaining was discarded. The residue was extracted with methanol for 2 more times. The sampled methanol extracts (3 mL) were combined and centrifuged at 13 000 rpm for 10 min to obtain the supernatant fractions that were frozen at -40 ℃ until analysis. The extract solution was appropriately diluted with methanol before analysis. Finally

a 10 μL aliquot was injected into the LC-MS/MS system

which was operated in the positive ion ESI mode with selected reaction monitoring for all the potential analytes. Result: Sixteen chemicals

including flavonoids and diarylheptanoids

were identified using LC-MS/MS with selected reaction monitoring mode(MRM). Twelve flavonoids were chrysin

pinocembrin

tectochrysin

apigenin

galangin

3-methylgalangin

acacetin

kaempferol

kaempferide

quercetin

isorhamnetin and rutin

respectively. Four diarylheptanoids included yakuchinone A

oxyphyllacinol

hexahydrocurcumin and hannokinol. Conclusion: Sixteen chemicals were identified using LC-MS/MS and some unknown diarylheptanoids need to be identified in the future.