Objective: To purify a glycopeptide named P-Ⅱ from Panax ginseng and investigated its properties. Method: The glycopeptide was extracted by 50% ethanol

precipitated with high-concentration ethanol

after decolorized by active carbon obtained crude ginseng glycopeptide

then purified by DEAE-Sepharose CL-6B column

washed successively with 0.2

0.5 and 1.0 mol·L-1 NaCl aqueous solution. Then using Sephadex G-150 column eluted with an aqueous solution of 0.2 mol·L-1 NaCl to give P-Ⅱ. The contents of neutral carbohydrate

uronic acid and peptide were determined using glucose

galacturonic acid and bovine serum albumin as the standards. The characterization of P-Ⅱ was analyzed by cellulose acetate membrane chromatography

Sephadex G-100 column chromatography

gas chromatography

and amino acid analysis. Result: P-Ⅱ was a homogneous glycopeptide. The contents of neutral carbohydrate

uronic acid

and peptide in P-Ⅱ were 50.2%

38.5%

and 1.9%

respectively. The molar ratio of Rha

Ara

Glc and Gal was 0.45: 1.15: 1.00: 2.36.It was consisted 16 kinds of amino acids including Asp

Fhr

and Ser. Conclusion: P-Ⅱ was a homogeneous glycopeptide

rather than a mixture of sugars and peptides

and laid the foundation for the study of structure-activity of ginseng glycopeptide.