Objective: To establish an HPLC method for simultaneous determination of six indexes in Tongmai pill

provide reference for quality evaluation. Method: An Agilent ZORBAX SB-Aq chromatographic column (4.6 mm×250 mm

5 μm) was used. The mobile phase was acetonitrile-methanol-0.1%H3PO4 with flow rate of 1 mL·min-1. Column temperature set at 35 ℃. The wavelengths were 260 nm for strychnine

brucine

paeoniflorin

hesperidin

and salvianolic acid B

and 316 nm for ferulic acid. Result: Strychnine

brucine

paeoniflorin

hesperidin

salvianolic acid B

and ferulic acid showed good linearity in the range of 0.037-1.097

0.039-1.176

0.075-2.25

0.01-0.30

0.113-3.384

0.225-6.72 μg

respectively. The average recoveries were 99.69%

100.12%

99.56%

99.98%

99.52%

and 99.69%. Conclusion: The established method is simple

accurate

and reproducible. It can be used for quality control of the Tongmai pill.