HPLC Fingerprint Spectrum of  Leaves

PENG Liang; CHEN Jie; LI Yi-guang; RAO Yi; WEI Hui-zhen; SUI Rong-chun; YUAN Gui-ping

doi:10.13422/j.cnki.syfjx.2015030063

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HPLC Fingerprint Spectrum of Leaves

更新时间：2024-01-04

- HPLC Fingerprint Spectrum of Leaves
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 3, Pages: 63-66(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015030063
  CLC： R284.1
- Published：2015
- 稿件说明：
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PENG Liang, CHEN Jie, LI Yi-guang, et al. HPLC Fingerprint Spectrum of Leaves[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 63-66.
DOI：

PENG Liang, CHEN Jie, LI Yi-guang, et al. HPLC Fingerprint Spectrum of Leaves[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 63-66. DOI： 10.13422/j.cnki.syfjx.2015030063.

摘要

目的: 建立猴耳环叶的HPLC指纹图谱。方法: 色谱条件为Odyssll C18色谱柱(4.6 mm×250 mm

5 μm);流动相甲醇-0.5%磷酸水梯度洗脱

柱温35 ℃

流速1 mL·min-1

检测波长273 nm

进样量10 μL。结果: 采用UPLC/DAD/Q-TOF-MS xevo G2 Q-Tof LC-MS对15个共有峰中的6个色谱峰进行初步归属

确定为没食子酸、木犀草苷、没食子酸乙酯、(-)-(2S)-5

5'-四羟基黄烷-7-没食子酸酯和7

4'-二-O-没食子酰基特利色黄烷、槲皮苷

10批猴耳环叶的相似度均在0.97以上。结论: 该方法符合方法学要求

可用于猴耳环叶的全面质量评价。

Abstract

Objective: To establish the HPLC fingerprint of Archidendron clypearia leaves. Method: The chromatographic fingerprint was obtained with Odyssll C18 column (4.6 mm×250 mm

5μm) and gradient eluted with methanol and 0.5% phosphoric acid. The column temperature was maintained at 35 ℃

and the flow rate was 1 mL·min-1. The detection wavelength was 273 nm.The injection volume was 10 μL. Result: Six chromatographic peaks among 15 common peaks were identified by UPLC/DAD/Q-TOF-MS xevo G2 Q-Tof LC-MS and be intended to be gallic acid

galuteolin

gallic acid ethyl ester

(-)-(2S)-5

5'-tetrahydroxyflavan-7-gallate

4'-O-di-gallyoltricetiflavan

quercitrin. The similarities of ten batches of Archidendron clypearia leaves were above 0.97. Conclusion: The method accordance with the requirements of the methodology and can be used for the full quality evaluation of Archidendron clypearia leaves.

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Related Institution

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