ZHOU Xin-xin, WANG Cai-xia. Mechanism of Buzhong Yiqi Tang in Treatment of Spleen Qi Deficiency Syndrome Amyosthenia in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 92-95.
DOI:
ZHOU Xin-xin, WANG Cai-xia. Mechanism of Buzhong Yiqi Tang in Treatment of Spleen Qi Deficiency Syndrome Amyosthenia in Rats[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 92-95. DOI: 10.13422/j.cnki.syfjx.2015030092.
Mechanism of Buzhong Yiqi Tang in Treatment of Spleen Qi Deficiency Syndrome Amyosthenia in Rats
Objective: To study the possible mechanism of Buzhong Yiqi Tang in treatment of spleen Qi deficiency syndrome amyosthenia in rats. Method: A combined method of combined techniques and overstrain were used to establish a spleen Qi deficiency model in rat. Cabbage 15-20 g was given to rats every day
3 mL pig grease was orally given to rats in every other day for 15 days. After a successful modeling
the rats were divided into normal control group
spleen Qi deficiency group
Buzhong Yiqi Tang treatment group(high dose
middle dose
and low dose of 7.10
3.55
1.78 g·kg-1 respectively)
and Buzhong Yiqi Tang was orally administrated for 10 days. The weight
rectal temperature
resistance to cold survival percentage and swimming time of rat were detected
and the expression of p-MLC was detected by Western blot
the value was compared before and after the treatment. Result: The weight
rectal temperature
resistance to cold survival percentage
swimming time and the expression of p-MLC in model group had a significant decrease compared to normal control group. The above mentioned indexes in middle dose and high dose groups of Buzhong Yiqi Tang had a significant increase compared to model group. Conclusion: Buzhong Yiqi Tang showed an up-regulation expression of p-MLC
which may be an mechanism for the treatment of spleen Qi deficiency syndrome amyosthenia in rats.
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