Effects of Total Flavonoe of  of Two Species in Guizhou on IR-related Factors in Type 2 Diabetes

LU Qi-zhen; ZHANG Jing; WANG Yue-yun; HUANG Cong; DENG Wei

doi:10.13422/j.cnki.syfjx.2015030096

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Effects of Total Flavonoe of of Two Species in Guizhou on IR-related Factors in Type 2 Diabetes

更新时间：2024-01-04

- Effects of Total Flavonoe of of Two Species in Guizhou on IR-related Factors in Type 2 Diabetes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 3, Pages: 96-99(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015030096
  CLC： R285.5
- Published：2015
- 稿件说明：
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LU Qi-zhen, ZHANG Jing, WANG Yue-yun, et al. Effects of Total Flavonoe of of Two Species in Guizhou on IR-related Factors in Type 2 Diabetes[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 96-99.
DOI：

LU Qi-zhen, ZHANG Jing, WANG Yue-yun, et al. Effects of Total Flavonoe of of Two Species in Guizhou on IR-related Factors in Type 2 Diabetes[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 96-99. DOI： 10.13422/j.cnki.syfjx.2015030096.

摘要

目的: 研究黔产粗毛淫羊藿与黔岭淫羊藿中总黄酮(TFE)对四氧嘧啶(ALX)诱发2型糖尿病模型小鼠胰岛素抵抗(IR)相关因子的影响。方法: 雄性小鼠100只

随机取10只为正常组

喂养基础饲料;其余的采用高脂饲料喂养小鼠6周

单次ip 2%ALX(200 mg·kg-1)

建立2型糖尿病小鼠模型。将糖尿病模型小鼠随机分为6组

每组10只

分别为模型组

二甲双胍(150 mg·kg-1)组

两种TFE分别按剂量100

50 mg·kg-1分组

ig给药14 d。于末次给药后取血

检测小鼠血清胰岛素(FINS)、C肽水平;检测小鼠空腹血糖(FBG)

注射胰岛素(Ins) 40 min后血糖(BG);计算胰岛素耐量(ITT)

胰岛素抵抗指数(HOMA-IR)和胰腺指数;观察小鼠胰腺形态学改变。结果: 与正常组比较

模型组小鼠血糖升高非常显著(P<0.01)

空腹血清中C肽

FINS有明显增加(P<0.05)

胰腺组织损伤极为严重(P<0.01)

胰腺指数降低非常显著(P<0.01)。与模型组相比

两种淫羊藿TFE均能够较明显地改善模型小鼠ITT( P<0.05)

降低模型小鼠血清C肽、FINS含量及HOMA-IR(P<0.05)

提高模型小鼠的胰腺指数(P<0.05)

明显地修复模型小鼠的胰腺组织损伤(P<0.05)。两种TFE同剂量组相比

以粗毛淫羊藿TFE作用较为显著(P<0.05

P<0.01)。结论: 两种黔产淫羊藿TFE对ALX诱生的2型糖尿病模型小鼠能增加胰岛素敏感性

较明显地改善模型小鼠胰岛素抵抗相关因子

保护受损的胰腺组织。以粗毛淫羊藿TFE作用较为明显

这可能与粗毛淫羊藿TFE中淫羊藿苷(ICA)含量高有较大关系。

Abstract

Objective: To study the effects of total flavonoe of Epimedium(TFE)from Epimedium acuminatum and E. Leptorrhizum grown in Guizhou on insulin resistance (IR)-related factors in mice induced by alloxan(ALX). Method: Hundred male mice were divided into normal group and model group. The mice in model group were fed with high-fat diet for 6 weeks

then single intraperitoneal injection of 2% ALX solution (200 mg·kg-1) was made to establish type 2 diabetes models.The modeling mice were randomly divided into 6 groups with 10 in each group

including metformin group(150 mg·kg-1)

E.acuminatum TFE group(100

50 mg·kg-1)

E.leptorrhizum TFE group(100

50 mg·kg-1)

respectively

once a day for 14 days. After 14 days

the animals were fasted for 12 hours

and killed. Then the level of serum insulin (FINS) and C peptide was determined.The blood sugar (FBG) and BG after injection of ins 40 min were detected. Insulin tolerance (ITT)

insulin resistance index (HOMA-IR) and pancreatic index were calculated. HE staining was used to observe the morphologic changes of pancreas in mice. Result: Compared with the normal group

blood sugar of the model group increased significantly(P<0.01)

C-peptide and FINS significantly increased(P<0.05)

pancreatic tissue injury was serious(P<0.01)

the pancreas index decreased significantly(P<0.01). Compared with model group

two TFE from E. acuminatum all obviously improved ITT(P<0.05)

pancreatic index(P<0.05)

reduced C-peptide

FINS and HOMA-IR(P<0.05) and obviously alleviated pancreatic tissue injury(P<0.05) in mice induced by ALX. TFE from E. acuminatum had more obvious effect(P<0.05

P<0.01). Conclusion: Two kinds of TFE from E. acuminatum have certain effect on the type 2 diabetes mice induced by ALX

and can increase insulin sensitivity

improve IR-related factors on the type 2 diabetes mice

and have a good protective effect on the pancreatic tissue injured. Especially

TFE from E.acuminatum has obvious effect. Epimedium's active ingredient on diabetes may has a greater relationship with icariin.

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