Objective: To discuss the effects of Jiagasong decoction (JGSD) on oxidative damaged liver cells and to explore its possible Nrf2/Bach1-ARE-antioxidant enzymes pathway mechanism. Method: The liver injury cells model was induced by 100 U·L-1 glucose oxidase (GO) and evaluated by MTT. The experiment included 9 groups:the control group

the model group

20% JGSD contained serum group

5 μmol·L-1 all-trans retinoic acid (ATRA) group

80 μmol·L-1 genstein group

80 μmol·L-1 tert-butylhydroquinone (t-BHQ) group

t-BHQ+20%JGSD contained serum group

ATRA + 20% JGSD contained serum group

genstein + 20%JGSD contained serum group. The activities of alanine aminotransferase (ALT)

aspartate aminotransferase (AST)

malonaldehyde (MDA)

glutathione peroxidase (GSH-Px) were tested. The location in cells and nucleus protein levels of Nrf2 and Bach1 were tested by immunofluorescence and Western blot. Result: Compared to the control group

ALT

AST and MDA contents incearsed

GSH-Px activity and cell proliferation decreased

and levels of Nrf2 and Bach1 increased in the model group (P<0.01). The above indexes had good improvement in JGSD-contained groups (P<0.05

P<0.01). Moreover

there was coordinated effects between JGSY and the other three agents on Nrf2 expression (P<0.01). Meanwhile

JGSD coule promote the Bach1 export from cell nuclear (P<0.01). Conclusion: JGSD could protect GO-induced cells damage from oxidative stress

and its antioxidation mechanism is related to regulating Nrf2 and Bach1 protein levels in nuclear.