Primary Research on Mechanism of Changweiqing in Treating Colon Cancer Invasion and Metastasis

WANG Guo-juan; YU Wen-yan; FAN Zhong-ze

doi:10.13422/j.cnki.syfjx.2015030142

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Primary Research on Mechanism of Changweiqing in Treating Colon Cancer Invasion and Metastasis

更新时间：2024-01-04

- Primary Research on Mechanism of Changweiqing in Treating Colon Cancer Invasion and Metastasis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 3, Pages: 142-145(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015030142
  CLC： R285
- Published：2015
- 稿件说明：
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WANG Guo-juan, YU Wen-yan, FAN Zhong-ze. Primary Research on Mechanism of Changweiqing in Treating Colon Cancer Invasion and Metastasis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 142-145.
DOI：

WANG Guo-juan, YU Wen-yan, FAN Zhong-ze. Primary Research on Mechanism of Changweiqing in Treating Colon Cancer Invasion and Metastasis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(3): 142-145. DOI： 10.13422/j.cnki.syfjx.2015030142.

摘要

目的: 肠胃清抗结肠癌侵袭转移的分子机制研究。方法: 以人结肠癌细胞株LoVo为研究对象

实验分为空白组

肠胃清低、中、高剂量组(0.98

1.97

3.93 g·L-1)

奥沙利铂组(9.23 mg·L-1)

联合组(肠胃清中剂量联合奥沙利铂组);药物作用于结肠癌LoVo细胞后

采用RT-qPCR法

Western blot法检测肠胃清对侵袭转移相关的E钙黏蛋白(E-cadherin)及Wint/β-链蛋白(β-catenin)信号通路因子β-catenin

(基质金属蛋白酶-7)MMP-7 mRNA及蛋白的影响。结果: 与空白组比较

肠胃清低、中、高剂量组、奥沙利铂组及联合组均升高LoVo细胞E-cadherin mRNA的表达(P<0.01)

均能降低β-catenin及MMP-7 mRNA的表达(P<0.05

P<0.01)

联合组作用更强;与空白组比较

肠胃清低、中、高剂量组、奥沙利铂组及联合组均升高LoVo细胞E-cadherin蛋白的表达(P<0.05)

均能降低β-catenin及MMP-7蛋白的表达(P<0.05

P<0.01)

联合组作用更强。结论: 肠胃清可以增加E-cadherin的表达量

增强细胞间的黏附能力

同时肠胃清可抑制Wint/βcatenin信号通路活性

降低β-catenin

MMP-7的表达

与奥沙利铂有协同作用。

Abstract

Objective: To explore the molecular mechanisms of Changweiqing in treating colon cancer invasion and metastasis. Method: The experiment included the blank group

the low-

medium-

high-dose Changweiqing groups (0.98

1.97

3.93 g·L-1)

the oxaliplatin group(9.23 mg·L-1)

and the combination group (1.97 g·L-1 Changweiqing plus 9.23 mg·L-1 oxaliplatin). The colon cancer LoVo cells were used in this study. The mRNA and protein of E-cadherin

Wint/beta chain protein signaling pathway factor β-catenin and matrix metalloproteinase-7 (MMP-7) were detected by RT-qPCR and Western blot assay. Result: Compared to the blank group

the mRNA

protein expression of E-cadherin increased

the mRNA expression of β-catenin and MMP-7 decreased in LoVo cells at all doses of Changweiqing groups

oxaliplatin group and combined group (P<0.05

P<0.01). Meanwhile

the results were better in the combination group. Conclusion: Changweiqing has synergetic effect with oxaliplatin by increasing the expression of E-cadherin and enhancing the adhesion between cells

inhibiting Wint/β-catenin signaling pathway activity

decreasing the expression of β-catenin and MMP-7.

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Related Institution

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