Objective: To optimize technological parameters of purification process of total balsamic acids from Benzoinum by anion exchange resin. Method: UV was adopted to determine the content of total balsamic acids with detection wavelength at 223 nm.With adsorption capacity and eluting ratio of total balsamic acids as indexes

three types(717

D211 and D301) of anion exchange resin was seleted by static adsorption-elution test

then technological parameters of purification process was optimized by single factor test. Result: Exchange capacity of 717 anion resin was better than the other two kinds of resins

optimal technological parameters were as follows:the concentration of sample solution 0.02 g · mL-1

pH of 12.0

sample volume of 8 BV

adsorption flow rate of 2 BV · h-1

removed impurities with 3 BV of water and 1 BV of dilute hydrochloric acid

eluted with 5 BV of 95% ethanol

elution flow rate of 1 BV · h-1.Under these conditions

purity of total balsamic acids could reached 73.49%

which increased 2.78 times by comparing with 26.47% before purification. Conclusion: Purification efficiency of 717 anion exchange resin for total balsamic acids from Benzoinum is good

and this optimized purification process is stable and feasible.