YUE Chao, GAO Jie, SHI Shang-mei, et al. Simultaneous Determination of Three Components in Crude and Processed Rehmanniae Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 71-74.
DOI:
YUE Chao, GAO Jie, SHI Shang-mei, et al. Simultaneous Determination of Three Components in Crude and Processed Rehmanniae Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 71-74. DOI: 10.13422/j.cnki.syfjx.2015040071.
Simultaneous Determination of Three Components in Crude and Processed Rehmanniae Radix by HPLC
比较炮制前后含量变化。 方法: Kromasil 100-5C18色谱柱(4.6 mm×250 mm
5 μm)
流动相乙腈(A)-水(B)进行梯度洗脱
流速1.0 mL ·min-1
检测波长203 nm
柱温30 ℃。 结果: 在该条件下3个活性成分均得到良好的分离
生地黄中
地黄苷D、益母草苷和毛蕊花糖苷分别在进样量68.10~3 405
99.40~4 970
14.52~726.0 ng有良好的线性关系
加样回收率在97.7%~99.0%。 结论: 建立的含量测定方法稳定、灵敏度高
重复性强
可以用于地黄的质量评价。
Abstract
Objective: To develop an HPLC method for simultaneous determination of rehmannioside D
leonuride
acteoside in crude and processed Rehmanniae Radix
then compared the content variation before and after processing. Method: Kromasil 100-5 C18 column (4.6 mm×250 mm
5 μm)
the mobile phase was a mixture of acetonitrile (A)-water (B) at a flow rate of 1.0 mL · min-1 for gradient elution
the column temperature was 30 ℃ with analysis was monitored at 203 nm. Result: Three components had good linear relationships:rehmannioside D in the range of 68.10-3 405 ng
leonuride in the range of 99.40-4 970 ng
acteoside in the range of 14.52-726.0 ng
the average recoveries in the range of 97.7%-99.0%. Conclusion: The method was steady with high precision and good sensitivity. It could be used for the quality control of Rehmanniae Radix.