Objective: To obtain high quality DNA from Curcuma kwangsiensis for simple sequence repeat(SSR) analysis

and provide a reference for research of genetic diversity of this variety. Method: Taking C. kwangsiensis leaves as experimental material

total DNA was extracted by the classic cetyl trimethyl ammonium bromide(CTAB) method and the modified CTAB method

difference between machine grinding and hand grinding was investigated

concentration and purity were determined by UV.DNA as a template for polymerase chain reaction(PCR) amplification

taking clest SSR-01

clest SSR-03

clest SSR-06

clest SSR-08

clest SSR-14 and clest SSR-15 as primers

polyacrylamide gel electrophoresis was adopted to detect DNA amplification effect. Result: When DNA extracted by the modified CTAB method

A260 nm/A280 nm of them was 1.8-2.0

proteins

polysaccharides

RNA and other substances were removed completely

DNA conformed to requirements of PCR amplification result

it could amplify out clear bands by C. universal primers and was suitable for SSR analysis.A260 nm/A280 nm of DNA extracted by the classic CTAB method was 1.5-1.6

it contained some impurities

which cloud not be used for PCR amplification and other molecular biology researches. Conclusion: DNA quality is good which was extracted by the modified CTAB method

this method can effectively remove interferenceof the secondary metabolites for DNA

it is suitable for SSR molecular marker and genetic diversity analysis analysis of C. kwangsiensis.