Neuroprotective Effect of Total Flavonoids of  on Parkinson Disease

YOU Jian-jun; LI Guang; LI Yu-chi; WANG Wan-li; LI Yi-hang

doi:10.13422/j.cnki.syfjx.2015040139

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Neuroprotective Effect of Total Flavonoids of on Parkinson Disease

更新时间：2024-01-04

- Neuroprotective Effect of Total Flavonoids of on Parkinson Disease
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 4, Pages: 139-143(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015040139
  CLC： R285.5
- Published：2015
- 稿件说明：
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YOU Jian-jun, LI Guang, LI Yu-chi, et al. Neuroprotective Effect of Total Flavonoids of on Parkinson Disease[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 139-143.
DOI：

YOU Jian-jun, LI Guang, LI Yu-chi, et al. Neuroprotective Effect of Total Flavonoids of on Parkinson Disease[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 139-143. DOI： 10.13422/j.cnki.syfjx.2015040139.

摘要

目的: 研究肾茶总黄酮对6-羟基多巴胺(6-OHDA)诱致帕金森病(Parkinson's disease

PD)大鼠模型及细胞模型的保护作用。方法: Wistar雄性大鼠80只随机分为造模组和正常组

造模组70只

正常组10只

除正常组外

采用定位注射6-OHDA(8 μg溶于4 μL含质量分数为0.2%抗坏血酸的生理盐水中)损毁大鼠单侧黑质多巴胺(DA)神经元的方法建立PD大鼠模型

将造模成功的大鼠随机分成5组

即模型组、肾茶总黄酮高、中、低剂量组(45

11 mg ·kg-1 ·d-1)、美多芭组(7.8 mg ·kg-1 ·d-1)

给药组ig给予相应药物

给药14 d

给药结束后

进行大鼠行为学检测并处死

采用ELISA法测定大鼠脑组织中丙二醛 (MDA)

过氧化氢酶 (CAT)

谷胱甘肽过氧化物酶(GSH-Px)

超氧化物歧化酶 (SOD)

DA和高香草酸(HVA)多项指标的水平。以神经母细胞瘤细胞(SH-SY5Y细胞)为研究对象

实验分为空白组、模型组、肾茶总黄酮2

0.5

0.25 g ·L-1 组

共6组

除空白组外

各组加入30 μmol ·L-1的6-OHDA

复制PD细胞模型

肾茶总黄酮各剂量组均经过不同浓度肾茶总黄酮预处理

继续培养20 h后

对细胞存活率和细胞形态进行观察比较。结果: 与正常组比较

模型组PD大鼠的旋转次数增加

脑组织CAT

GSH-Px

SOD和HVA水平明显降低

MDA水平明显升高

均具有统计学差异(P<0.01);与模型组比较

肾茶总黄酮高剂量可以明显减少PD大鼠旋转次数

肾茶总黄酮高、中、低剂量组均可不同程度的提高脑组织CAT

GSH-Px

SOD和HVA水平

降低MDA水平(P<0.05

P<0.01)

但是在改善神经行为和提高DA水平方面作用效果不及美多芭明显。与空白组比较

模型组6-OHDA引起的细胞损伤较明显(P<0.05);与模型组比较

肾茶总黄酮(2

1 g ·L-1)组预处理组可以明显减轻6-OHDA引起的细胞损伤(P<0.05

P<0.01)。结论: 肾茶总黄酮对6-OHDA诱导的PD大鼠模型和细胞模型具有明显的保护作用

其可能的作用机制与减少抗氧化应激引起的细胞损伤相关。

Abstract

Objective: To investigate the protective effect of total flavonoids of Clerodendranthus spicatus on Parkinson disease (PD) in rat and cell models. Method: The PD model was induced by positioning injection of 6-hydroxydopamine on right substantia nigra of rats. Seventy PD rats were randomly divided into 5 groups:the model group;the high-

middle-

low-dose total flavonoids of C. spicatus groups (45

11 mg · kg-1 · d-1);the madopar group (7.8 mg · kg-1 · d-1). Another 10 healthy rats were assigned to the control group. After 14 days of treatment

the levels of malondialdehyde (MDA)

catalase (CAT)

glutathion peroxidase (GSH-Px)

superoxide dismutase (SOD)

dopamine (DA) and 4-hydroxy-3-methoxyphenylacetic (HVA) in brain tissues of rats were detected by ELISA. SH-SY5Y cells were divided into the control group

the model group

and different dosage of total flavonoids of C. spicatus groups (2

0.5

0.25 g · L-1). The PD cell model was induced by adding 30 μmol ·L-1 6-OHDA. The survival rate and morphology were observed and compared after 20-h incubation. Result: Compared with the control group

rotating frequency of rats in PD model group increased

the levels of CAT

GSH-Px

SOD and HVA in brain tissue decreased significantly

MDA level increased with statistically significant difference (P<0.01). Compared with the model group

rotated times of PD rats decreased significantly in the high-dose total flavonoids of C. spicatus group (P<0.05)

the levels of CAT

GSH-Px

SOD and HVA increased

MDA concentration decreased in all doses of total flavonoids of C. spicatus groups (P<0.05

P<0.01). However

the improvement of neurological behavior and DA level in the total flavonoids of C. spicatus groups were not as good as madopar. Moreover

the total flavonoids of C. spicatus(2

1 g · L-1) pretreatment could reduce the cellular damage caused by 6-OHDA as compared with the control group(P<0.05

P<0.01). Conclusion: Total flavonoids of C. spicatus have obvious protective effect on the PD rat model and cell model induced by 6-OHDA. The protective effects may be related to reducing cell damage by resisting oxidative stress.

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