Objective: To observe growth inhibition of Huangqi injections on human immortalized cervical epithelial cells(H8 cells) and make research on cell cycle regulation mechanism. Method: Effect of Huangqi injections on H8 cells was detected by MTT

form of cells after being injected was observed on optical microscope

cells cycle of H8 was analyzed by flow cytometry(FCM).First antibodies of immunofluorescence assay were cyclins D1 and B1

cyclin-dependent kinases(CDK) 1 and 4

cyclin-dependent kinase inhibitor(p21)

second antibody was fluorescein labeled

fluorescence degree of H8 cells after being injected Huangqi injections was determined by FCM. Result: There was inhibiting effect on growth of H8 cells after being injected

function was the most obvious of 20 g · L-1.Some of the Huangqi injections treatment group appeared to be sparse in cell growth

death or apoptosis

cells stained pyknosis hyperchromatic circular corpuscle.When Huangqi injections on H8 cells

S and G2 phase cells decreased

cell blocked in Gl period.Expression of cyelin D1 protein in H8 cells significantly decreased;expression of cyclin B1

CDK4 and P21 were slightly reduced

but without statistical significance;expression of CDK1 protein reduced significantly. Conclusion: Huangqi injections can be regarded as a cell cycle regulation agent for prevention or adjuvant therapy of cervical cancer.