SHI Jun, LI Xin, ZHANG Xiao-ling, et al. Influence of Tongxie Yaofang with or Without Saposhnikoviae Radix Colonic Mucosa in Rats with Experimental Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 161-165.
DOI:
SHI Jun, LI Xin, ZHANG Xiao-ling, et al. Influence of Tongxie Yaofang with or Without Saposhnikoviae Radix Colonic Mucosa in Rats with Experimental Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 161-165. DOI: 10.13422/j.cnki.syfjx.2015040161.
Influence of Tongxie Yaofang with or Without Saposhnikoviae Radix Colonic Mucosa in Rats with Experimental Ulcerative Colitis
Objective: To observe the impact of Saposhnikoviae Radix of Tongxie Yaofang decoction on peroxisome proliferator-activated receptor (PPAR-γ) protein expression in the ulcerative colitis rats
and to explore the role of Saposhnikoviae Radix. Method: Sixty female SD rats were randomly divided into 6 groups
10 rats in each group. The rat ulcerative colitis(UC) model was made with 50% 2
4
6-trinitrobenzenesulfonic acid(TNBS)ethanol solution.The corresponding prescription without and with Saposhnikoviae Radix
half dose of Saposhnikoviae Radix were orally given
the dose was 18
20
22 g · kg-1
positive group was given salicylazosulfapyridine (SASP) at dose of 0.3 g · kg-1. The weight change
the colon mucosa damage index and colon pathology were observed
and immunohistochemical detection of PPAR-γ protein expression level was carried out. Result: The model group rats had colon mucosa inflammation and ulcer formation
compared with the blank group the result was statistically significant (P<0.05). Expression of PPAR-γ protein in model group rats colon tissue was lower than that in the control group
the difference was statistically significant (P<0.05).After the treatment
PPAR-γ protein expression level in Tongxie Yaofang decoction group was significantly higher than that in groups without or reduced Saposhnikoviae Radix (P<0.05). Conclusion: Saposhnikoviae Radix is minister drug in Tongxie Yaofang decoction
its dosage has significant impact on action of the decoction.
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