As an early isolated organ perfusion method

the isolated perfused kidney (IPK) has many advantages

such as the removal of nerves and humoral factors

convenience to exert interference factors and real-time monitoring the diversity of renal function. The IPK has great application value in studying drug metabolism

excretion and revelant mechanisms in kidney. This article aims to expand the domestic perspective of using IPK. This paper discussed the applications of IPK in the study of changes in kidney molecules

pathogenesis and interaction of various adjustment factors in nephropathy development process from many aspects

such as regulatory mechanisms of glomerular filtration

reabsorption and secretion of tubule and collecting duct

and renal ischemia injury model. By analyzing a large number of documents

we found that the using of IPK in foreign is relatively mature

while the domestic is more limited

mainly applied in metabolic excretion mechanism of drugs

mechanisms of drug-induced renal injury or donor kidney transplants

etc. This article described the use of IPK technology in the research of the molecular level changes in the kidney and renal mechanisms of pathogenesis. It could provide a reference for the domestic study of changes in renal function and mechanism stimulated by medication and risk factors. It also noted that IPK technology would be very important in morphological and functional changes of podocytes and its molecular mechanisms in the perspective of the whole kidney.