SUN Wen-jie, CHEN Yan, QU Ding, et al. Investigation of Key Parameters in Process of Removal of Starch in Coicis Semen by Enzymatic Hydrolysis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 16-19.
DOI:
SUN Wen-jie, CHEN Yan, QU Ding, et al. Investigation of Key Parameters in Process of Removal of Starch in Coicis Semen by Enzymatic Hydrolysis[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 16-19. DOI: 10.13422/j.cnki.syfjx.2015050016.
Investigation of Key Parameters in Process of Removal of Starch in Coicis Semen by Enzymatic Hydrolysis
Objective: To provide a reference for application of total polysaccharides in Coicis Semen by investigating effect of key parameters on high temperature α-amylase enzymolysis for starch in Coicis Semen. Method: Take the content of reducing sugar as a quantitative indicator and color change after iodine solution testing as a qualitative indicator
based on single factor tests
orthogonal test was adopted to investigate effects of the amount of enzyme
hydrolysis temperature
hydrolysis pH and reaction time on amylohydrolysis. Result: Optimal parameters were as follows:the amount of enzyme 600 U·g-1
enzymolysis temperature 75 ℃
enzymatic pH of 6.0
enzymolysis time of 120 min.The content of reducing sugar in extract of total polysaccharides in Coicis Semen was about 25%
meanwhile
color of samples did not alter after iodine solution testing.Purity of total polysaccharides in Coicis Semen was 76.63%
which showed a great improvement by comparing with 54.41% of samples without removing starch. Conclusion: High-temperature α-amylase can remove starch from Coicis Semen readily and rapidly
it is conducive to extraction of total polysaccharides.
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