Optimization of Clarification Process of Gastrodiae Rhizoma Extract with Chitosan Flocculating Method

TIAN Yang; SHI Chong-ying; HOU Yan; MU Ying-chao; LUO Yu; XIAO Rong

doi:10.13422/j.cnki.syfjx.2015050025

您当前的位置：

首页 >

文章列表页 >

Optimization of Clarification Process of Gastrodiae Rhizoma Extract with Chitosan Flocculating Method

更新时间：2024-01-04

- Optimization of Clarification Process of Gastrodiae Rhizoma Extract with Chitosan Flocculating Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 5, Pages: 25-28(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015050025
  CLC： R283.6
- Published：2015
- 稿件说明：
移动端阅览
TIAN Yang, SHI Chong-ying, HOU Yan, et al. Optimization of Clarification Process of Gastrodiae Rhizoma Extract with Chitosan Flocculating Method[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 25-28.
DOI：

TIAN Yang, SHI Chong-ying, HOU Yan, et al. Optimization of Clarification Process of Gastrodiae Rhizoma Extract with Chitosan Flocculating Method[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 25-28. DOI： 10.13422/j.cnki.syfjx.2015050025.

摘要

目的: 优选壳聚糖澄清天麻提取液的工艺条件

为该药材的临床应用提供参考。方法: 采用HPLC测定天麻素含量

流动相乙腈-水(3:97)

检测波长220 nm。以澄清效果和固形物含量为指标

在单因素试验基础上

通过正交试验考察壳聚糖添加量、药液浓缩比、絮凝温度、静置时间对天麻水提液壳聚糖絮凝工艺条件的影响。结果: 最佳工艺条件为壳聚糖添加量0.15%

药液浓缩比1:13 g·mL-1

絮凝温度25 ℃

静置时间10 h。天麻素质量浓度65.93 g·L-1

含固量3.157%。结论: 采用壳聚糖絮凝法澄清天麻提取液的方法简单易行、经济实用、澄清效果好

适用于中药水提液的精制。

Abstract

Objective: To optimize clarification process of Gastrodiae Rhizoma extract with chitosan flocculating method. Method: HPLC was employed to determine the content of gastrodin with mobile phase of acetonitrile-water(3: 97) and detection wavelength at 220 nm.Taking clarification effect and solids content as indexes

on the basis of single factor tests

orthogonal design was used to optimize clarification process with the adding amount of chitosan

liquid concentration ratio

flocculation temperature and standing time as factors. Result: Optimal clarification procedure was obtained under these conditions:the adding amount of chitosan 0.15%

liquid concentration ratio 1.13 g·mL-1

flocculation temperature at 25 ℃

standing time of 10 h.The concentration of gastrodin was 65.93 g·L-1

solids content was 3.157%. Conclusion: This optimized process is feasible and economical with good clarification effect

which is suitable for purification of Chinese medicine extract.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Gastrodin Inhibits Non-alcoholic Fatty Liver Disease via Mediating SREBP1c Signaling Pathway

Comparison of in Vitro Dissolution of Active Ingredients in Gastrodiae Rhizoma Powder with Different Particle Size

Study on Qualitative and Quantitative Methods of Fufangtianma Capsule

Study on the Composing Rule of Soup of Banxia, Baishu and Tianma

Related Author

ZHANG Menglian

ZHANG Yaowen

TANG Linfeng

GONG Zaipeng

HAN Lan

WANG Dandan

Fan LIU

Ting-ting CHEN

Related Institution

Anhui Province Key Laboratory of Traditional Chinese Medicine，School of Pharmacy， Anhui University of Chinese Medicine

State Key Laboratory of Efficacy and Utilization of Medicinal Plants，Guizhou Medical University

Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University

The Affiliated Hospital of Guizhou Medical University

Guangzhou University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰