Optimization of Strain N-14 Fermentation Process of Total Polysaccharides from Astragali Radix by Response Surface Methodology

GUO Yun-xia; WU Guo-jiang; WEI Meng; HAN Yu-jie; LIU Yue-qin; HAO Qing-hong

doi:10.13422/j.cnki.syfjx.2015050038

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Optimization of Strain N-14 Fermentation Process of Total Polysaccharides from Astragali Radix by Response Surface Methodology

更新时间：2024-01-04

- Optimization of Strain N-14 Fermentation Process of Total Polysaccharides from Astragali Radix by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 5, Pages: 38-41(2015)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015050038
  CLC： R284.2
- Published：2015
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GUO Yun-xia, WU Guo-jiang, WEI Meng, et al. Optimization of Strain N-14 Fermentation Process of Total Polysaccharides from Astragali Radix by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 38-41.
DOI：

GUO Yun-xia, WU Guo-jiang, WEI Meng, et al. Optimization of Strain N-14 Fermentation Process of Total Polysaccharides from Astragali Radix by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 38-41. DOI： 10.13422/j.cnki.syfjx.2015050038.

摘要

目的: 优化益生菌N-14发酵黄芪总多糖的工艺条件。方法: 采用UV测定总多糖含量

检测波长620 nm。在单因素试验基础上

以总多糖得率为响应值

底物添加量、发酵时间和灭菌时间为自变量

通过响应面试验优选黄芪总多糖发酵工艺。结果: 最佳发酵条件为底物添加量4 g

发酵时间24 h

灭菌时间15 min

pH 7

接种量1%。黄芪总多糖得率6.56%

与预测值6.69%偏差较小

较水提醇沉工艺的4.17%提高了57.31%。结论: 响应面分析法可用于优选益生菌N-14发酵黄芪的工艺条件

优选的工艺条件稳定可行

为该药材资源的充分利用提供参考。

Abstract

Objective: To optimize fermentation conditions of total polysaccharides from Astragali Radix by probiotic N-14. Method: UV was adopted to determine the content of total polysaccharides with detection wavelength at 620 nm.Based on single factor tests

response surface methodology(RSM) was employed to optimize fermentation process of total polysaccharides from Astragali Radix with yield of total polysaccharides as response value and sterilization time

fermentation time

the amount of substrate as independent variables. Result: Optimum fermentation conditions were as follows:the amount of substrate 4 g

fermentation time of 24 h

sterilization time of 15 min

pH of 7

inoculation of probiotic 1%.Yield of total polysaccharides was up to 6.56%

which was lower than the predictive value of 6.69%

but 57.31% higher than yield of total polysaccharides(4.17%) by water extraction and alcohol precipitation process. Conclusion: RSM is suited to optimize fermentation process of Astragali Radix with probiotic N-14

this optimized process is stable and feasible

it provides a reference for utilization of this herb.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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College of Pharmacy, Chengdu University of TCM

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