Optimization of Extraction Technology for Total Saponins from Ziziphi Spinosae Semen by Orthogonal Test

LIN Hai-cheng; ZHU Hong-yan; HE Zhong-mei; WANG Guo-li; ZHANG Lian-xue

doi:10.13422/j.cnki.syfjx.2015050042

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Optimization of Extraction Technology for Total Saponins from Ziziphi Spinosae Semen by Orthogonal Test

更新时间：2024-01-04

- Optimization of Extraction Technology for Total Saponins from Ziziphi Spinosae Semen by Orthogonal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 5, Pages: 42-45(2015)
- 作者机构：
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- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015050042
  CLC： R284.2
- Published：2015
- 稿件说明：
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LIN Hai-cheng, ZHU Hong-yan, HE Zhong-mei, et al. Optimization of Extraction Technology for Total Saponins from Ziziphi Spinosae Semen by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 42-45.
DOI：

LIN Hai-cheng, ZHU Hong-yan, HE Zhong-mei, et al. Optimization of Extraction Technology for Total Saponins from Ziziphi Spinosae Semen by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 42-45. DOI： 10.13422/j.cnki.syfjx.2015050042.

摘要

目的: 优选酸枣仁总皂苷的提取工艺

为该成分的工业化生产提供参考。方法: 采用HPLC-UV检测酸枣仁皂苷A和B的含量

流动相乙腈-水(34:66)

检测波长204 nm。在单因素试验基础上

通过正交试验考察乙醇体积分数、乙醇用量、提取时间及提取次数对酸枣仁总皂苷提取工艺的影响。结果: 酸枣仁皂苷A

B的线性范围分别为0.24～2.40

0.12～1.20 μg

平均加样回收率分别为100.39%

99.40%。酸枣仁总皂苷最佳提取工艺为加12倍量70%乙醇回流提取2次

每次1.5 h;酸枣仁皂苷A

B的平均质量分数分别为0.606

0.228 mg·g-1。结论: 建立的HPLC检测酸枣仁皂苷A

B含量方法准确可靠

优选的酸枣仁总皂苷提取工艺稳定可行

具有节约成本、省时等特点

适用于工业化生产。

Abstract

Objective: To optimize extraction technology of total saponins from Ziziphi Spinosae Semen and provide a reference for industrial production of this component. Method: HPLC-UV method was employed to detect contents of jujuboside A and jujuboside B with mobile phase of acetonitrile-water(34: 66) and detection wavelength at 204 nm.On the basis of single factor tests

effects of ethanol concentration

liquid-sodid ratio

extraction time and extraction times on extraction process were investigated by orthogonal test. Result: Linear ranges of jujuboside A and jujuboside B were 0.24-2.40

0.12-1.20 μg

average recovery of them were 100.39% and 99.40%.The best extraction technology of total saponins from Ziziphi Spinosae Semen was as following:extracted 2 times with 12 times the amount of 70% ethanol for 1.5 h per time;average contents of jujuboside A and jujuboside B were 0.606

0.228 mg·g-1

respectively. Conclusion: This established HPLC method is accurate and reliable to detect contents of jujuboside A and jujuboside B.At the same time

this optimized extraction technology of total saponins from Ziziphi Spinosae Semen is stable and feasible with advantages of energy-saving

time-saving and so on

so it is suitable for industrial production.

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