Objective: To optimize extraction process of burning Drynariae Rhizoma formula granules and establish determination of naringin in this preparation. Method: Taking extracting amount of naringin and yield of dry extract as a comprehensive evaluation index

orthogonal test was adopted to optimize extraction process with the amount of water

extraction time and extraction times as factors.Naringin in this formula granules was determinated by HPLC

mobile phase was methanol-acetic acid-water(35: 4: 65) and detection wavelength was 283 nm. Result: The best extraction technique was as follows:soaked 0.5 h

extracted 2 times with 10

8 times the amount of water for 0.5 h per time.The mass fraction of naringin was 0.77% and yield of dry extract was 12.6%.Calibration curve of naringin was linear in the range of 0.063-1.575 μg

average recovery was 100.70% with RSD of 1.2%.Tentatively per 1 g of this formula particle contained ≥5 mg of naringin. Conclusion: This extraction process and determination are stable and feasible with high extraction efficiency of naringin

which can be scientific basis for production and quality control of burning Drynariae Rhizoma formula granules.