Simultaneous Determination of Five Nucleosides in Bailing Capsule by HPLC

ZHENG Yu-qian; CHENG Qiao-yuan; ZHU Ming; ZHANG Yun

doi:10.13422/j.cnki.syfjx.2015050061

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Simultaneous Determination of Five Nucleosides in Bailing Capsule by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Five Nucleosides in Bailing Capsule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 5, Pages: 61-64(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015050061
  CLC： O657.72;R286.0
- Published：2015
- 稿件说明：
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ZHENG Yu-qian, CHENG Qiao-yuan, ZHU Ming, et al. Simultaneous Determination of Five Nucleosides in Bailing Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 61-64.
DOI：

ZHENG Yu-qian, CHENG Qiao-yuan, ZHU Ming, et al. Simultaneous Determination of Five Nucleosides in Bailing Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 61-64. DOI： 10.13422/j.cnki.syfjx.2015050061.

摘要

目的: 建立HPLC同时测定百令胶囊中5种核苷类成分尿苷酸、尿苷、腺苷酸、鸟苷和腺苷含量的方法。方法: 采用Ultimate AQ C18色谱柱(4.6 mm×250 mm

5 μm)

以甲醇-0.05 mol·L-1磷酸二氢钾溶液为流动相进行梯度洗脱

流速1.0 mL·min-1

检测波260 nm

柱温25 ℃。结果: 在该色谱条件下

尿苷酸、尿苷、腺苷酸、鸟苷和腺苷分离良好

且分别在35.25~528.8(r=1.000)

38.07~571.1(r=1.000)

34.96~524.5(r=0.999 8)

38.34~575.1(r=1.000)

39.84~597.6 ng (r=1.000)线性关系良好。加样回收率分别为101.2%

103.1%

96.7%

101.4%

103.2%

RSD分别为1.9%

1.1%

1.3%

2.7%

1.6%。结论: 该HPLC含量测定方法结果准确、可靠

具有良好的专属性、精密度、重复性和稳定性

可用于百令胶囊的质量控制。

Abstract

Objective: To establish an HPLC method for simultaneous determination of five nucleosides

uridine 5'-monophosphate

uridine

adenosine 5'-monophosphate monohydrate

guanosine

adenosine in Bailing capsules. Method: The separation was developed on an Ultimate AQ C18 column (4.6 mm×250 mm

5 μm) by gradient elution with methanol-0.05 mol·L-1 potassium dihydrogen phosphate solution

with flow rate at 1.0 mL·min-1 and column temperature at 25 ℃. The detection wavelength was at 260 nm. Result: The baseline separation of uridine 5'-monophosphate

uridine

adenosine 5'-monophosphate monohydrate

guanosine

and adenosine was successfully achieved. The results showed that these five ingredients had good linearity within the test ranges of 35.25-528.8(r=1.000)

38.07-571.1(r=1.000)

34.96-524.5(r=0.999 8)

38.34-575.1(r=1.000) and 39.84-597.6 ng(r=1.000)

respectively. The recovery were 101.2% (RSD 1.9%)

103.1% (RSD 1.1%)

96.7% (RSD 1.3%)

101.4% (RSD 2.7%) and 103.2% (RSD 1.6%)

respectively. Conclusion: The developed method of the HPLC-fingerprint and quantitative analysis showed good specificity

precision

reproducibility

stability and could be used for the quality control of Bailing capsules.

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