Influence of Irradiation Sterilization on Chemical Constituents of Scutellariae Radix

ZHANG Qiu-mei; SUN Zeng-yu

doi:10.13422/j.cnki.syfjx.2015050072

您当前的位置：

首页 >

文章列表页 >

Influence of Irradiation Sterilization on Chemical Constituents of Scutellariae Radix

更新时间：2024-01-04

- Influence of Irradiation Sterilization on Chemical Constituents of Scutellariae Radix
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 5, Pages: 72-74(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015050072
  CLC： R284.1
- Published：2015
- 稿件说明：
移动端阅览
ZHANG Qiu-mei, SUN Zeng-yu. Influence of Irradiation Sterilization on Chemical Constituents of Scutellariae Radix[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 72-74.
DOI：

ZHANG Qiu-mei, SUN Zeng-yu. Influence of Irradiation Sterilization on Chemical Constituents of Scutellariae Radix[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 72-74. DOI： 10.13422/j.cnki.syfjx.2015050072.

摘要

目的: 用高效液相色谱法对经辐照灭菌的黄芩药材、黄芩药材粉末及未经灭菌黄芩药材中黄芩苷的含量进行测定。比较灭菌前后黄芩药材及其粉末中黄芩苷含量变化情况。方法: 采用Accurasil-C18色谱柱(4.6 mm×250 mm

5 μm);流动相甲醇-水-磷酸(47:53:0.2)

检测波长280 nm

流速1.0 mL·min-1

柱温30 ℃

进样量10 μL。结果: 黄芩苷在0.163 2~0.816 0 μg线性关系良好(r2= 0.999 8)

平均回收率为99.44%

RSD 1.6%。灭菌前后饮片与粉末中黄芩苷含量均有显著性变化。结论: 以黄芩苷含量为指标考察

表明黄芩药材不适宜应用辐照灭菌法进行灭菌。

Abstract

Objective: HPLC was adopted to determine the baicalin content of the Scutellariae Radix and Scutellariae Radix powder by using irradiation sterilization

together with herbs without the sterilization. Compared the content changes before and after mirradiation sterilization. Method: Accurasil-C18 chromatographic column(4.6 mm×250 mm

5 μm)

methanol-water-phosphoric acid (47: 53: 0.2) as mobile phase

the wavelength detection was 280 nm with flow rate of 1.0 mL·min-1

column temperature 30 ℃

and the injection volume of 10 μL. Result: Baicalin had good linear relationship in the range of 0.163 2-0.816 0 μg. The average recovery was 99.44% with the RSD of 1.6%. Content baicalin in Radix had significant changes before and after sterilization. Conclusion: It indicates that Scutellariae Radix is not suitable for applications of irradiation sterilization

which takes baicalin as index.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Optimization of Electromagnetic Cracking Extraction Technology of Baicalin in Scutellariae Radix by Response Surface Methodology

Screening and Identification of Endophytic Fungi in Scutellariae Radix Transformed Baicalin to Baicalein and Oroxylin A

Determination of Chlorogenic Acid、Baicalin and Phillyrin in Shuanghuanglian in-situ Gel by HPLC

Related Author

Ren-guang WANG

Ai-ling JIA

Zhi-dong QIU

Jing-yao YANG

Xin-shu ZHANG

Ji-qi YANG

Zong-min MA

Jia LIU

Related Institution

Key Laboratory of Active Ingredients in Traditional Chinese Medicine(TCM) Co-constructed by Jilin Province and Ministry of Education, Changchun University of Chinese Medicine

College of Pharmacy, Chengdu University of TCM

Hebei Key Laboratory of Turbiditytoxin Syndrome, Hebei Academy of Chinese Medical Sciences

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences

Nanning institute for Food and Drug Control of Guangxi

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰