Simultaneous Determination of Eight Components in Compound Huangkui Granules by HPLC

JIANG Jing-yi; LIU Huan; WANG Li-ling; LIU Zhi-hui

doi:10.13422/j.cnki.syfjx.2015050095

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Eight Components in Compound Huangkui Granules by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Eight Components in Compound Huangkui Granules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 5, Pages: 95-98(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015050095
  CLC： O657.72;R286.0
- Published：2015
- 稿件说明：
移动端阅览
JIANG Jing-yi, LIU Huan, WANG Li-ling, et al. Simultaneous Determination of Eight Components in Compound Huangkui Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 95-98.
DOI：

JIANG Jing-yi, LIU Huan, WANG Li-ling, et al. Simultaneous Determination of Eight Components in Compound Huangkui Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 95-98. DOI： 10.13422/j.cnki.syfjx.2015050095.

摘要

目的: 建立HPLC同时测定复方黄葵颗粒中毛蕊异黄酮葡萄糖苷、金丝桃苷、芦丁、异槲皮苷、杨梅素、槲皮素、马钱苷、莫诺苷的方法。方法: 采用Aglient C18色谱柱(4.6 mm×250 mm

5 μm)

以乙腈-0.05%甲酸水溶液为流动相进行梯度洗脱

流速 0.8 mL·min-1

检测波长分别为240

260

360 nm

柱温 30 ℃。结果: 毛蕊异黄酮葡萄糖苷、金丝桃苷、芦丁、异槲皮苷、杨梅素、槲皮素、莫诺苷、马钱苷线性范围分别为3.5～35.0(r=0.999 6)

32.09～320.9(r=0.999 7)

2.21～22.12(r=0.999 7)

18.68～186.9(r=0.999 8)

6.32～63.20(r=0.999 7)

3.54～35.44(r=0.999 6)

12.39～123.9(r=0.999 6)

12.88～128.8 mg·L-1(r=0.999 6)。平均回收率分别为98.1% (RSD 1.7%)

99.7%(RSD 0.6%)

98.7%(RSD 2.5%)

100.1%(RSD 1.4%)

99.7%(RSD 1.0%)

99.9%(RSD 2.7%)

99.0%(RSD 1.2%)

99.7%(RSD 1.3%)。结论: 所建立方法简便、准确

重复性好

可作为复方黄葵颗粒的质量控制。

Abstract

Objective: To establish an HPLC method for simultaneously determining the contents of calycosin-7-O-β-D-glucoside

hypericin

rutin

isoquercetin

myricetin

quercetin

loganin

morroniide in compound Huangkui granules. Method: The determination was performed on an Aglient C18(4.6 mm×250 mm

5 μm) chromatographic column

the mobile phase was acetonitrile-0.05% formic acid aqueous solution with gradient elution at a flow rate of 1.0 mL·min-1;the detection wavelength was 240

260 and 360 nm

column temperature was 30 ℃. Result: The calibration curves of calycosin-7-O-β-D-glucoside

hypericin

rutin

isoquercetin

myricetin

quercetin

loganin

morroniide were linear in the ranges of 3.5-35.0(r=0.999 6)

32.09-300.9(r=0.999 7)

2.21-22.12(r=0.999 7)

18.68-186.9(r=0.999 8)

6.32-63.20(r=0.999 7)

3.54-35.44(r=0.999 6)

12.39-123.9(r=0.999 6)

12.88-128.8 mg·L-1(r=0.999 6)

respectively. The average recoveries were 98.1%(RSD 1.7%)

99.7%(RSD 0.6%)

98.7%(RSD 2.5%)

100.1%(RSD 1.4%)

99.7%(RSD 1.0%)

99.9%(RSD 2.7%)

99.0%(RSD 1.2%)

99.7%(RSD 1.3%)

respectively. Conclusion: The method established is simple

accurate and reproducible

which can be used for the quality control of Huangkui granules.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Simultaneous Determination of Calycosin-7--glucoside and Astragaloside Ⅳ in Astragali Radix by HPLC

Comparison of Flavonoid Components in Astragali Radix and its Processed Products

Flavonoids Fingerprint of One or Two Years Old Hedysari Radix and Astragali Radix

Comparison of Flavonoid Components in Astragali Radix and its Processed Products

Investigation of Component Difference of Astragali Radix Before and After Rice Stir-frying by UPLC-Q-TOF-MS/MS Combined with Chemometrics

Related Author

SONG Cheng-ying

FENG Jia-fu

SONG Xiao-wei

LI Qing

YE Jing

ZHANG Yuan-ting

CHEN Xiao-hui

BI Kai-shun

Related Institution

School of Pharmacy,Shenyang Pharmaceutical University

Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine

Nanjing Women and Children's Healthcare Hospital

Jiangsu Haibin Rehabilitation Hospital

Xiyuan Hospital， China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰