LI Jin, QIN Yong-chang, LI Gui-yong, et al. Acute Toxicity Test of Extracts from Stalks and Leaves of Coix[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 178-181.
DOI:
LI Jin, QIN Yong-chang, LI Gui-yong, et al. Acute Toxicity Test of Extracts from Stalks and Leaves of Coix[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(5): 178-181. DOI: 10.13422/j.cnki.syfjx.2015050178.
Acute Toxicity Test of Extracts from Stalks and Leaves of Coix
Objective: The aim of this article was to explore the acute toxicity of water extracts and alcohol extracts from stalks and leaves of Coix on mice so as to determine the median lethal dose (LD50) and the maximum tolerated dose (MTD). Method: Seventy-four KM mice (half male and half female) of grade SPF were divided into extract group and control group. After fasted for 16 hours before experiment
all the mice were fed by gavage. The toxic reaction and death were oberved. If the mice died
it will be dissected to observe the changes of heart
liver
spleen
lung
kidney
brain
stomach
small intestine and other organs by eyes.After the observation for 14 days
the survivals in each group were killed
and then dissected. Then we examined their main pathological changes in organs by eyes as mentioned above. Result: No obvious symptoms of poisoning were discovered on these mice
neither death. Conclusion: The maximum tolera dose of SPF KM mice to the water extracts and the alcohol extracts from leaves of Coix
and to the water extract and alcohol extracts from stalks of Coix
were 42.08
34.80
39.52
32.64 g·kg-1
respectively
with all greater than 5 g·kg-1. According to the quarter experiment
the water and alcohol extracts from leaves and stalks of Coix are nontoxic.
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Related Author
Yu-ping XIA
Chun-hua HE
Zi-shu CHAI
Wen-hui QIN
Wen-xin WU
Liu-yan CHEN
Jia-li LIU
Mei RU
Related Institution
Guangxi University of Chinese Medicine
Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica
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Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine