ZHU Cheng, PENG Guo-ping, LI Cun-yu, et al. Purification and Qualitative Analysis of Total Phenolics from Leaves[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 29-31.
DOI:
ZHU Cheng, PENG Guo-ping, LI Cun-yu, et al. Purification and Qualitative Analysis of Total Phenolics from Leaves[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 29-31. DOI: 10.13422/j.cnki.syfjx.2015060029.
Purification and Qualitative Analysis of Total Phenolics from Leaves
Objective: The extraction and purification methods for phenolics in Morus alba leaves were studied and the constituents of phenolic were identified
provided suitable experimental drug specimen for exploring the hypoglycemic effect of M. alba leaves. Method: The optimum extraction condition was investigated by orthogonal design and the extraction quantity was regarded as the investigated index. The factors affecting separation
such as resin dosage
pH and concentration of eluting agent were considerate. The chemical components of phenolic extracted from M. alba were analyzed and identified by UPLC-MS. Result: The optimized extracting conditions were as follows:extracted two times with 70% alcohol
each 1.5 h. The ratio of solvent to material 10:1 and 8:1
respectively;and the purification process with macroporous resin HPD-826 was using 1%Na2CO3
60% ethanol to wash out phenolics;eight phenolics compounds were identified by UPLC-MS. Conclusion: This method is simple
short cycle
low cost
high content and suitable for industrialized production. This purification technology could be used to extract phenolics of M. alba leaves for studies on its hypoglycemic effect.
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Related Author
MIN Yun-jiang1
ZHOU Shou-biao2
ZHANG Yin-ping1
GAO Qiong1
ZHANG Xiao-lei1
WEI Wei
JIN Ri-xian
CHEN Yan-jun
Related Institution
School of Pharmacy,Nanchang University
Key Laboratory of Modern Preparation of Traditional Chinese Medicine
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