YUAN Rong, SUN Li-li, YANG Li-wei, et al. Fingerprint of Keteling Capsules and Content Determination of Main Components by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 37-40.
DOI:
YUAN Rong, SUN Li-li, YANG Li-wei, et al. Fingerprint of Keteling Capsules and Content Determination of Main Components by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 37-40. DOI: 10.13422/j.cnki.syfjx.2015060037.
Fingerprint of Keteling Capsules and Content Determination of Main Components by UPLC
Objective: To establish the fingerprint of the Keteling capsules and content determination of vitexin and isovitexin by UPLC. Method: The Acquity UPLC HSS C18(2.1 mm×100 mm
1.7 μm)column was used
the column temperature was 25 ℃
the mobile phase consisted of methanol-0.05% formic acid with gradient elution. The flow rate was 0.2 mL · min-1
and the detection wavelength was 325 nm. Fingerprint Similarity Evaluation Software(edition 2012.130723) was used to calculate the similarity and Acquity Waters system was used for the assay. Result: The fingerprint of the Keteling capsules was established
13 characteristic peaks were identified and the content of vitexin and isovitexin were determined. Conclusion: Established the UPLC fingerprint and content determination methods and reproducibility with good precision and stability
which could manifest the overall quality characteristics of the Keteling capsules. It could provide a scientific basis for the production and quality control of the Keteling capsules.