Objective: To establish a method for simultaneous determination of catechin

chlorogenic acid

crocin-Ⅰ

and crocin-Ⅱ in Yinchenhao decoction by HPLC-DAD. Method: The Symmetry C18 column (4.6 mm×250 mm

5 μm) was used

the mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL · min-1. The column temperature was kept at 30 ℃ and the wavelength was set at 202 nm for catechin

330 nm for chlorogenic acid

and 440 nm for crocin-Ⅰand crocin-Ⅱ

respectively. Result: The linear range of catechin

chlorogenic acid

crocin-Ⅰ

and crocin-Ⅱ were 0.18-0.90 (r=0.999 8)

0.020 8-0.374 4 (r=0.999 9)

0.147-2.646 (r=0.999 9) and 0.007 84-0.141 12 (r=0.999 9) μg

respectively. The mean recovery of these 4 components were 98.56% (RSD 1.8%)

99.07% (RSD 1.8%)

97.41% (RSD 1.9%)

and 98.46% (RSD 1.9%)

respectively. Conclusion: This method is simple

accurate and specific

and can be used for the determination of 4 constituents in Yinchenhao decoction.