Comparison on Chemical Constituents from Different Size of Yacon Leaves

SUN Yun-chao; WANG Wei; WANG Xiao-tong; LI De-wei; RAN Xiao-ku; DOU De-qiang; DONG Feng

doi:10.13422/j.cnki.syfjx.2015060052

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Comparison on Chemical Constituents from Different Size of Yacon Leaves

更新时间：2024-01-04

- Comparison on Chemical Constituents from Different Size of Yacon Leaves
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 6, Pages: 52-56(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015060052
  CLC： R284.1
- Published：2015
- 稿件说明：
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SUN Yun-chao, WANG Wei, WANG Xiao-tong, et al. Comparison on Chemical Constituents from Different Size of Yacon Leaves[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 52-56.
DOI：

SUN Yun-chao, WANG Wei, WANG Xiao-tong, et al. Comparison on Chemical Constituents from Different Size of Yacon Leaves[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 52-56. DOI： 10.13422/j.cnki.syfjx.2015060052.

摘要

目的: 比较不同大小亚贡叶的高效液相指纹图谱

并对亚贡叶中绿原酸和沼菊素进行含量测定。方法: 采用Kromasil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.4%磷酸水

梯度洗脱(0~60 min

5%~85%乙腈)

流速1.0 mL ·min-1

检测波长0~18 min

327 nm

18.1~60 min

210 nm;进样量10 μL

柱温30 ℃。结果: 建立不同大小亚贡叶指纹图谱

较大的亚贡叶样品绿原酸含量高(1.588 2 mg ·g-1)

较小的亚贡叶样品沼菊素含量高(5.637 4 mg ·g-1)。结论: 亚贡叶样品中绿原酸、沼菊素含量与叶子大小有相关性。该方法操作简便

结果准确

重复性好

实验结果可用于指导亚贡叶的质量控制。

Abstract

Objective: To establish an HPLC fingerprint of different size of yacon leaves

and determine the content of chlorogenic acid and enhydrin. Method: The fingerprints were obtained on a Kromasil C18 (4.6 mm×250 mm

5 μm) with the gradient elution solvent system composed of acetonitrile and 0.4% phosphoric acid. The flow rate was 1.0 mL · min-1. The column temperature was maintained at 30 ℃

and the detection wavelength was 0-18 min

327 nm

18.1-60 min

210 nm. Result: The fingerprint of yacon leaves was established. Larger leaves samples with high content of chlorogenic acid(1.588 2 mg · g-1)

and the small yacon leaves with high content of enhydrin(5.637 4 mg · g-1). Conclusion: The chlorogenic acid and enhydrin content in yacon leaves was correlated with the size. This method is easy to operate with the high exactitude and the excellent repetition for the quantitative analysis of yacon leaves.

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