Simultaneous Determination of Tanshinone Ⅱ, Tanshinone Ⅰ, Cryptotanshinone and Dihydrotanshinone Ⅰ in Salviae Albae Radix by HPLC

ZHANG Lin-lin; WANG Chun; SONG Zhi-qian; DU Zhi-yong; NING Zhang-chi; DONG Yun-zhuo; LIU Zhen-li

doi:10.13422/j.cnki.syfjx.2015060062

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Simultaneous Determination of Tanshinone Ⅱ, Tanshinone Ⅰ, Cryptotanshinone and Dihydrotanshinone Ⅰ in Salviae Albae Radix by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Tanshinone Ⅱ, Tanshinone Ⅰ, Cryptotanshinone and Dihydrotanshinone Ⅰ in Salviae Albae Radix by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 6, Pages: 62-65(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015060062
  CLC： O657.72;R284.2
- Published：2015
- 稿件说明：
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ZHANG Lin-lin, WANG Chun, SONG Zhi-qian, et al. Simultaneous Determination of Tanshinone Ⅱ, Tanshinone Ⅰ, Cryptotanshinone and Dihydrotanshinone Ⅰ in Salviae Albae Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 62-65.
DOI：

ZHANG Lin-lin, WANG Chun, SONG Zhi-qian, et al. Simultaneous Determination of Tanshinone Ⅱ, Tanshinone Ⅰ, Cryptotanshinone and Dihydrotanshinone Ⅰ in Salviae Albae Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 62-65. DOI： 10.13422/j.cnki.syfjx.2015060062.

摘要

目的: 建立同时测定白花丹参中丹参酮ⅡA、丹参酮Ⅰ、隐丹参酮和二氢丹参酮Ⅰ含量的方法。方法: 从白花丹参产地山东采集了10个批次白花丹参

采用HPLC测定4种主要丹参酮成分丹参酮ⅡA、丹参酮Ⅰ、隐丹参酮和二氢丹参酮Ⅰ含量。色谱条件:Zorbax Extend-C18色谱柱(4.6 mm×150 mm

5 μm)

流动相乙腈-0.2%乙酸水(57:43)

流速1.0 mL ·min-1

检测波长270 nm

柱温30 ℃。结果: 丹参酮ⅡA、丹参酮Ⅰ、隐丹参酮和二氢丹参酮Ⅰ分别在0.138～2.76(r=0.999 9)

0.024 8 ～0.496(r=0.999 9)

0.096～1.92(r=0.999 9)和0.035～0.70 μg(r=0.999 9)线性关系良好。加样回收率分别为100.06%

99.60%

99.91%

100.06%。丹参酮ⅡA、丹参酮Ⅰ、隐丹参酮和二氢丹参酮Ⅰ含量范围分别是2.677～7.150

0.320～1.747

0.916～5.935

0.132～2.601 mg ·g-1。结论: 该法简便、准确、分离度好

能同时测定白花丹参中4种有效成分的含量。

Abstract

Objective: To establish the method for simultaneous determination of tanshinone ⅡA

tanshinone I

cryptotanshinone and dihydrotanshinone I in Salviae Albae Radix by HPLC. Method: Each of ten batches of Salviae Albae Radix were collected from the same place-Shandong province. A simple and effective high-performance liquid chromatographic (HPLC) method has been developed for simultaneous quantification of four main diterpenes (tanshinone IIA

tanshinone I

cryptotanshinone

dihydrotanshinone I) in Salviae Albae Radix. Chromatography was performed on a (4.6 mm×150 mm

5 μm) particle size

extend-C18 column. The mobile phase was a linear gradient prepared from 0.2% aqueous formic acid and acetonitrile (43:57) at a flow-rate of 1.0 mL · min-1. Detection was accomplished at 270 nm and column temperature was set at 30 ℃. Result: The contents of tanshinone IIA

tanshinone I

cryptotanshinone

dihydrotanshinone I in Salviae Albae Radix were between 2.677-7.150

0.320-1.747

0.916-5.935

0.132-2.601 mg · g-1

repectively. The average recovery was 100.06%

99.60%

99.91%

100.06%

repectively. Conclusion: The developed method is simple

accurate and good separation

which is suitable for the quality control of tanshinone ⅡA

tanshinone I

cryptotanshinone and dihydrotanshinone I in Salviae Albae Radix.

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