HAO Zhen-feng, LIU Jing, ZHANG Yu, et al. Effects of Shikonin on Proliferation, Invasion and Migration in Cervical Cancer Hela Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 91-94.
DOI:
HAO Zhen-feng, LIU Jing, ZHANG Yu, et al. Effects of Shikonin on Proliferation, Invasion and Migration in Cervical Cancer Hela Cells[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 91-94. DOI: 10.13422/j.cnki.syfjx.2015060091.
Effects of Shikonin on Proliferation, Invasion and Migration in Cervical Cancer Hela Cells
Objective: To investigate the effect of shikonin on proliferation
invasion and migration of human cervical cancer Hela cells and to explore the possible mechanism. Method: The human cervical cancer Hela cells were cultivated and divided into control group and shikonin group (the concentration was respectively 4
8
16
32
64 μmol · L-1). The effect of shikonin on cell viability
apoptosis
invasion and migration of cells cultivated after 24 h were studied by MTT assay
flow cytometric and Transwell assay
respectively. When cells were cultivated after 24 h
the expression of focal adhesion kinase(FAK) protein
the level of phosphorylated FAK
and mRNA were assessed by Western blot and quantitative real-time PCR analysis. Result: Shikonin inhibited proliferation and induced apoptosis of Hela cells in a dose-dependent manner. When treated with low-toxic doses of shikonin (4
8
16 μmol · L-1)
the cell invasion and migration were markedly suppressed. Furthermore
shikonin significantly down-regulated the expression of FAK and the level of phosphorylated FAK. In addition
shikonin could increase the expression of FAK mRNA. Conclusion: Shikonin inhibits proliferation and invasion of cervical cancer Hela cells by inhibiting phosphorylation of FAK.
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