ZHONG Mi-cun, SU Xiao-hui, KONG Xiang-ying, et al. Effects of Tetramethylpyrazine on Migration and Tube Formation of BMECs[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 103-107.
DOI:
ZHONG Mi-cun, SU Xiao-hui, KONG Xiang-ying, et al. Effects of Tetramethylpyrazine on Migration and Tube Formation of BMECs[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 103-107. DOI: 10.13422/j.cnki.syfjx.2015060103.
Effects of Tetramethylpyrazine on Migration and Tube Formation of BMECs
Objective: To observe the effects of tetramethylpyrazine(TMP) on the proliferation
migration
tube formation and secretion of the stromal cell-derived factor-1(SDF-1) and vascular endothelial cell growth factor(VEGF) from brain microvascular endothelial cells(BMECs). Method: Primary isolated BMECs of passage 3 to 6 were used in this experiment. BMECs that treated with TMP(2
10
50 mg · L-1) were set as TMP 2
TMP 10 and TMP 50 groups
and that treated with the same volume of medium was regarded as control. Effects of TMP on the proliferation of BMECs were detected by MTS and BrdU incorporation. Impact of TMP on migration of BMECs was observed by Transwell assay. The influence of TMP on tube formation of BMECs induced by VEGF was observed by tube formation in vitro. The level of SDF-1 and VEGF in BMECs culture supernatant were examined by ELISA. Result: BrdU incorporation and MTS results showed that TMP(2
10
50 mg · L-1) can significantly increase the proliferation of BMECs(P<0.05
P<0.01). TMP can dose-dependently promote BMECs migrate to the lower chamber of Transwell and increase the number of branch points in tube formation. In addition
TMP(2
10
50 mg · L-1) can significantly increase the level of SDF-1 and VEGF in cell culture supernatant (P<0.05
P<0.01). Conclusion: TMP could effectively promote BMECs proliferation
migration and tube formation. Meanwhile
it could increase the secretion of SDF-1 and VEGF from BMECs
which may be part of the mechanism for promoting angiogenesis.
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