Effect of Ziyin Qianyang Formula on IP3, DAG Mediated by AT1 Receptors in Renal Hypertensive Rats' Peripheral Blood Lymphocytes

LI Yuan-yuan; LIU Na; LIAO Sheng-bao; DAI Min

doi:10.13422/j.cnki.syfjx.2015060140

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Effect of Ziyin Qianyang Formula on IP3, DAG Mediated by AT1 Receptors in Renal Hypertensive Rats' Peripheral Blood Lymphocytes

更新时间：2024-01-04

- Effect of Ziyin Qianyang Formula on IP3, DAG Mediated by AT1 Receptors in Renal Hypertensive Rats' Peripheral Blood Lymphocytes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 6, Pages: 140-144(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015060140
  CLC： R285.5
- Published：2015
- 稿件说明：
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LI Yuan-yuan, LIU Na, LIAO Sheng-bao, et al. Effect of Ziyin Qianyang Formula on IP3, DAG Mediated by AT1 Receptors in Renal Hypertensive Rats' Peripheral Blood Lymphocytes[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 140-144.
DOI：

LI Yuan-yuan, LIU Na, LIAO Sheng-bao, et al. Effect of Ziyin Qianyang Formula on IP3, DAG Mediated by AT1 Receptors in Renal Hypertensive Rats' Peripheral Blood Lymphocytes[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 140-144. DOI： 10.13422/j.cnki.syfjx.2015060140.

摘要

目的: 探讨高血压神经-内分泌-免疫调节的内在机制和滋阴潜阳方作用的可能环节。方法: SD大鼠120只

复制二肾一夹高血压大鼠模型(2K1C-RHR)

假手术组大鼠右肾动脉只分离不狭窄;取模型成功大鼠分为模型组、滋阴潜阳方高、中、低剂量组(32

8 g ·kg-1)、氯沙坦钾组(0.005 g ·kg-1)及天麻钩藤颗粒组(1.5 g ·kg-1)

连续ig给药4周。采用无创血压测量仪观察其对血压的影响

放射性免疫法检测血浆血管紧张素Ⅱ(AngⅡ)含量变化

Western bloting法测定各组大鼠外周血淋巴细胞血管紧张素1型受体(AT1R)和兔抗磷酸化蛋白激酶C(p-PKC)的表达水平变化

ELISA法测定各组大鼠外周血淋巴细胞内三磷酸肌醇(IP3)

二酰甘油(DAG)以及血清中白细胞介素-4(IL-4)

白细胞介素-6(IL-6)含量变化。结果: 与假手术组比较

模型组大鼠血压明显升高

大鼠血浆AngⅡ水平明显升高

大鼠外周血淋巴细胞AT1R

p-PKC蛋白的表达明显升高

外周血淋巴细胞内IP3

DAG含量明显升高

血清中IL-4

IL-6的含量明显升高

均具有统计学差异(P<0.05

P<0.01);与模型组比较

滋阴潜阳方高、中、低剂量组均能显著降低模型大鼠血压水平(P<0.01)

滋阴潜阳方中、低剂量组能够降低大鼠血浆AngⅡ水平(P<0.05)

高剂量组能够降低肾性高血压大鼠外周血淋巴细胞AT1R

p-PKC蛋白的表达水平(P<0.01

P<0.05)

同时高剂量组能够明显降低外周血淋巴细胞内IP3

DAG(P<0.01

P<0.01)以及血清中IL-4

IL-6的含量(P<0.05

P<0.01)。结论: 肾性高血压病的发病机制可能与外周血淋巴细胞AT1R的过度表达

以及胞内磷脂酰肌醇信号通路的激活有关;滋阴潜阳方可以从多环节抑制信号通路的激活

对高血压的神经、内分泌、免疫功能发挥整体调节作用。

Abstract

Objective: To explore the immuno-neurologic regulation of renal hypertensive and its inherent law

and to investigate the mechanisms of curing and systemic regulating effect of Ziyin Qianyang formula. Method: Renal hypertension rats (RHR) model was established by two kidney one clip (2K1C) and 2K1C-RHR were divided into six groups:the model group

the high-

medium-

low- dose Zinyin Qianyang formula groups(32

8 g · kg-1)

the losartan potassium group (0.005 g · kg-1) and the Tianma Gouteng particles group (1.5 g · kg-1). Drugs were given by intragastric administration for 4 weeks. The blood pressure was measured by a non-invasive blood pressure monitor. The contents of plasma angiotensin Ⅱ (AngⅡ) were detected by radioimmunity method. The protein levels of angiotensin Ⅱ type l receptor (AT1R) and rabbit-anti protein kinase C (p-PKC) were detected by Western blotting. The contents of inositol triphosphate(IP3) and diacylglycerol(DAG) in peripheral blood lymphocytes and the levels of interlenkin-4(IL-4)

IL-6 in the blood serum of RHR were detected using ELISA. Result: Compared with the model group

Ziyin Qianyang formula could decrease blood pressure of RHR significantly (P<0.01). The medium-

low-dose Ziyin Qianyang formula could decrease the concentration of plasma AngⅡ (P<0.05). The high-

medium-dose Ziyin Qianyang formula could decrease the concentration of AT1R and p-PKC in peripheral blood lymphocytes of rats (P<0.05

P<0.01). The high-dose Ziyin Qianyang formula could decrease the concentration of IP3 and DAG (P<0.05

P<0.01) in peripheral blood lymphocytes and the levels of IL-4

IL-6 in blood serum of RHR (P<0.05

P<0.01). Conclusion: The renal hypertensive may be related to the excessive expression of AT1R in peripheral blood lymphocytes and the activation of IP3/DAG signaling pathway

where Ziyin Qianyang formula could exert immuno-neurologic regulation action.

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