WANG Yi, ZHANG Yan, LI Hai. Mechanism of Action Research on Yiqi Huoxue Decoction Intervene PGC-1 Control Heart Failure Myocardial Cell Energy Metabolismrefactoring[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 169-173.
DOI:
WANG Yi, ZHANG Yan, LI Hai. Mechanism of Action Research on Yiqi Huoxue Decoction Intervene PGC-1 Control Heart Failure Myocardial Cell Energy Metabolismrefactoring[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 169-173. DOI: 10.13422/j.cnki.syfjx.2015060169.
Mechanism of Action Research on Yiqi Huoxue Decoction Intervene PGC-1 Control Heart Failure Myocardial Cell Energy Metabolismrefactoring
Objective: The aim of this article was to observe the effect of Yiqi Huoxue decoction on myocardial energy metabolism of rats with congestive heart failure. Method: Eighty rats were subject to exhaustive swimming and hunger to cause heart failure model with coronary artery ligation.Two weeks after operation
the survivals were divided into four groups:model group
Chinese medicine group
western medicine group
and normal group 4 group
treated by gavage or with distilled water for 28 days. We detected the changes of the expression of proteins and genes of peroxisome proliferator activation receptor gamma coactivator 1 (PPAR γ-coactivator-1
PGC-1) by using Western blot and RT-PCR method.The concentration of high-energy phosphate (adenosine triphosphate
ATP) was determined by using colorimetric method
and the contents of serum N terminal pro brain natriuretic peptide (NT proBNP) and cardiac troponin I (cTNI) concentration were determined via ELISA. Result: Compared with normal group
the ATP and PGC-1 α levels of rats in model group and other groups were significantly lower (P<0.01)
while the contents of NT-proBNP and cTnI were significantly increased (P<0.01);compared with the model group
the ATP and PGC-1 α levels of rats in Chinese medicine group and western medicine group
increased significantly (P<0.01)
the contents of NT-proBNP and cTnI decreased (P<0.01). In western medicine group and Chinese medicine group
there was no significant difference inthe levels of ATP
PGC-1 α
NT-proBNP and cTnI in the serum. Conclusion: Yiqi Huoxue decoction can significantly reduce NT-proBNP and cTnI content in rats with chronic heart failure
improve the energy metabolism
promote the production of PGC-1 alpha
and delay the development of chronic heart failure.
Clinical Efficacy and Mechanism of Xiao Qinglongtang in Treating Chronic Heart Failure with Cold Phlegm in Lung
Effect of Xinshen Shuangbu Qiangxin Decoction on Plasma BNP and Related Index of Heart Function in Chronic Heart Failure Patients
Effect of Buxin Decoction on Content of B-type Natriuretic Peptide, Troponin I, Adenosine Triphosphate in Rats with Chronic Heart Failure after Myocardial Infarction
Mechanism of Yiqi Huoxue Decoction in Treating Coronary Heart Disease with Qi Deficiency and Blood Stasis Syndrome
Effect of Sijunzi Tang on Proteonomics of Myocardial Tissues In Rats With Chronic Heart Failure
Related Author
Yong-cheng WANG
Guo-feng ZHOU
Rui LIN
Wen-ting WANG
Si-jia WU
Feng DONG
Xiao LI
Zhao-yu LI
Related Institution
Shandong University of Traditional Chinese Medicine(TCM),Ji'nan
Affiliated Hospital of Shandong University of TCM,Ji'nan